Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

SMAD4 antibody

Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Catalog # M00074-1). Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00074-1
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

View all SMAD4 Antibodies

SKU/Catalog Number

M00074-1

Size

100uL

Form

Liquid

Description

Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Catalog # M00074-1). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00074-1)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM277

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the C-terminus of human Smad4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology.

Reactive Species

M00074-1 is reactive to SMAD4 in Human

Applications

M00074-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

60.439kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:500-1:1000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For SMAD4 (Source: Uniprot.org, NCBI)

Gene Name

SMAD4

Full Name

Mothers against decapentaplegic homolog 4

Weight

60.439kDa

Superfamily

dwarfin/SMAD family

Alternative Names

DPC4; DPC4SMAD, mothers against DPP homolog 4 (Drosophila); hSMAD4; JIP; MAD homolog 4; MADH4; MADH4mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD 4; SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4; Smad4 SMAD4 DPC4, JIP, MADH4, MYHRS SMAD family member 4 mothers against decapentaplegic homolog 4|MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic, Drosophila, homolog of, 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMAD4, check out the SMAD4 Infographic

SMAD4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMAD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00074-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression

Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis

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16 Customer Q&As for Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

Question

We are currently using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-09

Question

Is a blocking peptide available for product anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1)?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

We do provide the blocking peptide for product anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-05

Question

I was wanting to use your anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 for IHC for rat muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat muscle identification?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

As indicated on the product datasheet, M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-25

Question

I see that the anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 works with IHC, what is the protocol used to produce the result images on the product page?

E. Johnson

Verified customer

Asked: 2019-09-04

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-04

Question

We ordered your anti-Smad4 Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

I have checked the website and datasheets of our anti-Smad4 Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-07-10

Question

Is this M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 reactive to the isotypes of SMAD4?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The immunogen of M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 is A peptide corresponding to the C-terminus of human Smad4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-28

Question

Do you have a BSA free version of anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 available?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-17

Question

Our lab want to know about using your anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 for regulation of transforming growth factor beta receptor signaling pathway studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

Thank you for your inquiry. This M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 is tested on imr32 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-04-10

Question

My colleagues were happy with the WB result of your anti-Smad4 Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-03-19

Answer

Based on literature, placenta does express SMAD4. Generally SMAD4 expresses in cytoplasm. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2019-03-19

Question

Does anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 work for IHC with muscle?

S. Li

Verified customer

Asked: 2019-03-13

Answer

According to the expression profile of muscle, SMAD4 is highly expressed in muscle. So, it is likely that anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 will work for IHC with muscle.

Boster Scientific Support

Answered: 2019-03-13

Question

Would M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-07

Answer

As indicated on the product datasheet, M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-07

Question

Please see the WB image, lot number and protocol we used for muscle using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1. Let me know if you need anything else.

N. Krishna

Verified customer

Asked: 2015-07-14

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-07-14

Question

Can you help my question with product M00074-1, anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Bhatt

Verified customer

Asked: 2014-07-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-07-11

Question

We want to test anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 on rat muscle for research purposes, then I may be interested in using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Jones

Verified customer

Asked: 2014-07-02

Answer

The products we sell, including anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-07-02

Question

We have seen staining in rat liver. What should we do? Is anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 supposed to stain liver positively?

S. Evans

Verified customer

Asked: 2013-05-13

Answer

From literature liver does express SMAD4. From Uniprot.org, SMAD4 is expressed in kidney, fetal brain, placenta, muscle, liver, among other tissues. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2013-05-13

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Lead time for this item is typically 1-2 weeks

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M00074-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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