Anti-NOTCH2 Antibody Picoband™

Notch-2 antibody

Boster Bio Anti-NOTCH2 Antibody Picoband™ catalog # A00518-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00518-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-NOTCH2 Antibody Picoband™

View all Notch-2 Antibodies

SKU/Catalog Number

A00518-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOTCH2 Antibody Picoband™ catalog # A00518-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NOTCH2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00518-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NOTCH2 recombinant protein (Position: H2194-A2471).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00518-2 is reactive to NOTCH2 in Human, Mouse, Rat

Applications

A00518-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

265.405kDa

Background of Notch-2

Neurogenic locus notch homolog protein 2 also known as notch 2 is a protein that in humans is encoded by the NOTCH2 gene. It is mapped to 1p12. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For NOTCH2 (Source: Uniprot.org, NCBI)

Gene Name

NOTCH2

Full Name

Neurogenic locus notch homolog protein 2

Weight

265.405kDa

Superfamily

NOTCH family

Alternative Names

AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2 NOTCH2 AGS2, HJCYS, hN2 notch receptor 2 neurogenic locus notch homolog protein 2|Notch homolog 2|notch 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOTCH2, check out the NOTCH2 Infographic

NOTCH2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOTCH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00518-2

100μg

$370
A00518-2-10ug

10μg sample (liquid)

$99
A00518-2-Biotin

100 μg Biotin conjugated

$570
A00518-2-Cy3

100 μg Cy3 conjugated

$570
A00518-2-Dylight488

100 μg Dylight488 conjugated

$570
A00518-2-Dylight550

100 μg Dylight550 conjugated

$570
A00518-2-Dylight594

100 μg Dylight594 conjugated

$570
A00518-2-FITC

100 μg FITC conjugated

$570
A00518-2-HRP

100 μg HRP conjugated

$570
A00518-2-APC

100 μg APC conjugated

$670
A00518-2-PE

100 μg PE conjugated

$670
A00518-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A00518-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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