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- Table of Contents
SMAD4 Antibodies
AND SMAD4 ELISA kits, SMAD4 Proteins
Many biological assays use antibodies to detect SMAD4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SMAD4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SMAD4 antibodies...
Designing a Western blot for SMAD4? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SMAD4 Western Blot GuideSMAD4 Infographic by Boster Bio
All SMAD4 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00074-2
20μL, 40μL, 100μL, 200μL
Cat # M00074-2
20μL, 40μL, 100μL, 200μL
Human, Mouse, Rat
ELISA
$480
Cat # EKC1530
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1530
1 kit, containing one 96-well plate and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2278
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2278
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2277
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2277
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2276
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2276
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2275
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2275
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2274
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2274
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC2273
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2273
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse
ELISA
$540
Cat # EKC2272
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2272
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC1960
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC1960
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC1959
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC1959
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$540
Cat # EKC1958
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC1958
1 kit, containing two 96-well plates and all necessary reagents
SMAD4 Overview
Facts about Mothers against decapentaplegic homolog 4.
Mothers against decapentaplegic homolog 4 (SMAD4)
In muscle structure, plays a central role in the equilibrium between atrophy and hypertrophy. MSTN reduction causes SMAD4 discharge and subsequent recruitment by the BMP pathway to promote hypertrophy through phosphorylated SMAD1/5/8.
Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity).
Gene Information
| Human | |
|---|---|
| Gene Name: | SMAD4 |
| Uniprot: | Q13485 |
| Entrez: | 4089 |
Family
| Belongs to: |
|---|
| dwarfin/SMAD family |
Alternative Names
DPC4; DPC4SMAD, mothers against DPP homolog 4 (Drosophila); hSMAD4; JIP; MAD homolog 4; MADH4; MADH4mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD 4; SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4; Smad4
Molecular Weight
Mass (kDA):
60.439 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 18q21.2 |
| Sequence: | 18; NC_000018.10 (51030213..51085042) |
Subcellular Localizations
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Pancreatic Neoplasm
- Adenocarcinoma
- Malignant Neoplasm Of Pancreas
- Cell Transformation, Neoplastic
- Colorectal Cancer
- Neoplasm Metastasis
- Carcinogenesis
- Colorectal Neoplasms
- Polyposis
- Malignant Paraganglionic Neoplasm
Interacting Proteins
- AKT1
- DACH1
- DCP1A
- DLX4
- FOXO3
- FOXO4
- GATA2
- LMO4
- NLK
- NR2F2
Pathways
- Cell Proliferation
- Cell Growth
- Localization
- Pathogenesis
- Cell Cycle
- Secretion
- Cell Differentiation
- Angiogenesis
- Cell Migration
- Cell Death
- Methylation
- Wound Healing
- Cell Cycle Arrest
PTMs
- Phosphorylation
- Methylation
- Ubiquitination
- Acetylation
- Sumoylation
- Dephosphorylation
- Cleavage
- Reduction
Research Areas
- Signal Transduction
- Stem Cell Signaling Pathway
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMAD4
bosterbio.com/bosterbio-gene-info-cards/SMAD4
References
PMID: 8553070 by Hahn S.A., et al. DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1.
PMID: 8774881 by Zhang Y., et al. Receptor-associated Mad homologues synergize as effectors of the TGF- beta response.
7 Publications of Boster SMAD4 Antibodies & ELISA Kits
- PubMed ID: -, Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial�...
- PubMed ID: 25371235, TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhi...
- PubMed ID: 19824107, Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepat...
- PubMed ID: 27019660, Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in P...
- PubMed ID: 25483171, Molecular responses of radiation-induced liver damage in rats
- PubMed ID: 22549392, Chen Wh, Kong Xy, Wan R, Xiao Cs, Li L, Wang Zy, Lin N, Wang Hm. Chin J Integr M...
- PubMed ID: 24746831, Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. ...
*More publications can be found for each product on its corresponding product page