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Product Info Summary
| SKU: | M01625 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-SNAP25 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01625
BM5167 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SNAP25 Rabbit Monoclonal Antibody catalog # M01625. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SNAP25 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01625)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABHE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SNAP25
Reactive Species
M01625 is reactive to SNAP25 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
23.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01625 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: mouse brain tissue, mouse Neuro-2a whole cell
IHC: Hela cell, SH-SY5Y cell, MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of SNAP25 using anti-SNAP25 antibody (M01625).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified monoclonal antibody (M01625) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-SNAP25 Rabbit Monoclonal Antibody (M01625)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-SNAP25 Rabbit Monoclonal Antibody
Question
Is this M01625 anti-SNAP25 Rabbit Monoclonal antibody reactive to the isotypes of SNAP25?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of M01625 anti-SNAP25 Rabbit Monoclonal antibody is A synthesized peptide derived from human SNAP25. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
I have attached the WB image, lot number and protocol we used for fetal brain temporal cortex using anti-SNAP25 Rabbit Monoclonal antibody M01625. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
We ordered your anti-SNAP25 Rabbit Monoclonal antibody for IP on brain in the past. I am using mouse, and I plan to use the antibody for WB next. My question regards examining brain as well as fetal brain temporal cortex in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
I took a look at the website and datasheets of our anti-SNAP25 Rabbit Monoclonal antibody and it seems that M01625 has been validated on mouse in both IP and WB. Thus M01625 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-08
Question
Is there a BSA free version of anti-SNAP25 Rabbit Monoclonal antibody M01625 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SNAP25 Rabbit Monoclonal antibody M01625 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-SNAP25 Rabbit Monoclonal antibody for WB for rat fetal brain temporal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain temporal cortex identification?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
As indicated on the product datasheet, M01625 anti-SNAP25 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain temporal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
We have been able to see staining in human skeletal muscle. Are there any suggestions? Is anti-SNAP25 Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
From literature skeletal muscle does express SNAP25. From Uniprot.org, SNAP25 is expressed in frontal cortex, fetal brain temporal cortex, skeletal muscle, amygdala hippocampus, brain, eye, among other tissues. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2019-04-24
Question
I see that the anti-SNAP25 Rabbit Monoclonal antibody M01625 works with WB, what is the protocol used to produce the result images on the product page?
D. Brown
Verified customer
Asked: 2019-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain temporal cortex using anti-SNAP25 Rabbit Monoclonal antibody M01625. Let me know if you need anything else.
E. Kulkarni
Verified customer
Asked: 2019-03-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-04
Question
We are currently using anti-SNAP25 Rabbit Monoclonal antibody M01625 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The anti-SNAP25 Rabbit Monoclonal antibody (M01625) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-04
Question
I have a question about product M01625, anti-SNAP25 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Krishna
Verified customer
Asked: 2018-07-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01625 anti-SNAP25 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-04
Question
Does M01625 anti-SNAP25 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
You can see on the product datasheet, M01625 anti-SNAP25 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
I am interested in to test anti-SNAP25 Rabbit Monoclonal antibody M01625 on rat fetal brain temporal cortex for research purposes, then I may be interested in using anti-SNAP25 Rabbit Monoclonal antibody M01625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
The products we sell, including anti-SNAP25 Rabbit Monoclonal antibody M01625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-19
Question
We were well pleased with the WB result of your anti-SNAP25 Rabbit Monoclonal antibody. However we have observed positive staining in eye perinuclear region using this antibody. Is that expected? Could you tell me where is SNAP25 supposed to be expressed?
V. Roberts
Verified customer
Asked: 2015-09-09
Answer
According to literature, eye does express SNAP25. Generally SNAP25 expresses in cytoplasm, perinuclear region. Regarding which tissues have SNAP25 expression, here are a few articles citing expression in various tissues:
Amygdala, and Hippocampus, Pubmed ID: 14702039
Brain, Pubmed ID: 11780052
Eye, Pubmed ID: 15489334
Fetal brain, and Temporal cortex, Pubmed ID: 8112622
Frontal cortex, Pubmed ID: 8056350
Skeletal muscle, Pubmed ID: 8760387
Boster Scientific Support
Answered: 2015-09-09
Question
Does anti-SNAP25 Rabbit Monoclonal antibody M01625 work for WB with fetal brain temporal cortex?
Z. Walker
Verified customer
Asked: 2015-06-11
Answer
According to the expression profile of fetal brain temporal cortex, SNAP25 is highly expressed in fetal brain temporal cortex. So, it is likely that anti-SNAP25 Rabbit Monoclonal antibody M01625 will work for WB with fetal brain temporal cortex.
Boster Scientific Support
Answered: 2015-06-11
Question
Is a blocking peptide available for product anti-SNAP25 Rabbit Monoclonal antibody (M01625)?
T. Li
Verified customer
Asked: 2014-02-07
Answer
We do provide the blocking peptide for product anti-SNAP25 Rabbit Monoclonal antibody (M01625). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-02-07
