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Product Info Summary
| SKU: | M00758-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SOX10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00758-2
BM4665 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOX10 Rabbit Monoclonal Antibody catalog # M00758-2. Tested in WB, IHC application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00758-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GIE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX10
Reactive Species
M00758-2 is reactive to SOX10 in Human
Observed Molecular Weight
70 kDa
Calculated molecular weight
49.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00758-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
Positive Control
IHC: human breast tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human breast, using SOX10 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-SOX10 Rabbit Monoclonal Antibody (M00758-2)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-SOX10 Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-2. Let me know if you need anything else.
T. Brown
Verified customer
Asked: 2020-05-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
We are currently using anti-SOX10 Rabbit Monoclonal antibody M00758-2 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The anti-SOX10 Rabbit Monoclonal antibody (M00758-2) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-17
Question
I see that the anti-SOX10 Rabbit Monoclonal antibody M00758-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-19
Question
Our lab want to know about using your anti-SOX10 Rabbit Monoclonal antibody for neural crest cell migration studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We appreciate your inquiry. This M00758-2 anti-SOX10 Rabbit Monoclonal antibody is tested on mouse brain. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-06
Question
Our team were content with the WB result of your anti-SOX10 Rabbit Monoclonal antibody. However we have been able to see positive staining in small intestine cytoplasm. using this antibody. Is that expected? Could you tell me where is SOX10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
From literature, small intestine does express SOX10. Generally SOX10 expresses in cytoplasm. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-05
Question
My question regarding product M00758-2, anti-SOX10 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00758-2 anti-SOX10 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-14
Question
Is there a BSA free version of anti-SOX10 Rabbit Monoclonal antibody M00758-2 available?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SOX10 Rabbit Monoclonal antibody M00758-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-28
Question
We have seen staining in rat inferior olivary complex. Are there any suggestions? Is anti-SOX10 Rabbit Monoclonal antibody supposed to stain inferior olivary complex positively?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
According to literature inferior olivary complex does express SOX10. According to Uniprot.org, SOX10 is expressed in inferior olivary complex, small intestine, placenta skin, among other tissues. Regarding which tissues have SOX10 expression, here are a few articles citing expression in various tissues:
Placenta, and Skin, Pubmed ID: 15489334
Small intestine, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-07-24
Question
We ordered your anti-SOX10 Rabbit Monoclonal antibody for WB on small intestine in the past. I am using rat, and We are going to use the antibody for IHC next. We need examining small intestine as well as inferior olivary complex in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
I have checked the website and datasheets of our anti-SOX10 Rabbit Monoclonal antibody and it seems that M00758-2 has been tested on rat in both WB and IHC. Thus M00758-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-02
Question
Is a blocking peptide available for product anti-SOX10 Rabbit Monoclonal antibody (M00758-2)?
G. Zhao
Verified customer
Asked: 2015-11-09
Answer
We do provide the blocking peptide for product anti-SOX10 Rabbit Monoclonal antibody (M00758-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-11-09
Question
Is this M00758-2 anti-SOX10 Rabbit Monoclonal antibody reactive to the isotypes of SOX10?
K. Bhatt
Verified customer
Asked: 2014-07-16
Answer
The immunogen of M00758-2 anti-SOX10 Rabbit Monoclonal antibody is A synthesized peptide derived from human SOX10 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-16
Question
Will M00758-2 anti-SOX10 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Carter
Verified customer
Asked: 2013-12-12
Answer
As indicated on the product datasheet, M00758-2 anti-SOX10 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-12
Question
you antibody to test anti-SOX10 Rabbit Monoclonal antibody M00758-2 on human small intestine for research purposes, then I may be interested in using anti-SOX10 Rabbit Monoclonal antibody M00758-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Williams
Verified customer
Asked: 2013-09-12
Answer
The products we sell, including anti-SOX10 Rabbit Monoclonal antibody M00758-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-09-12
Question
I have attached the WB image, lot number and protocol we used for small intestine using anti-SOX10 Rabbit Monoclonal antibody M00758-2. Please let me know if you require anything else.
N. Li
Verified customer
Asked: 2013-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-19
Question
Will anti-SOX10 Rabbit Monoclonal antibody M00758-2 work for IHC with small intestine?
J. Edwards
Verified customer
Asked: 2013-06-12
Answer
According to the expression profile of small intestine, SOX10 is highly expressed in small intestine. So, it is likely that anti-SOX10 Rabbit Monoclonal antibody M00758-2 will work for IHC with small intestine.
Boster Scientific Support
Answered: 2013-06-12
Question
I was wanting to use your anti-SOX10 Rabbit Monoclonal antibody for IHC for human small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine identification?
G. Krishna
Verified customer
Asked: 2013-03-20
Answer
It shows on the product datasheet, M00758-2 anti-SOX10 Rabbit Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-20
