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- Table of Contents
SOX2 antibody (M00105-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IF analysis of SOX2 using anti-SOX2 antibody (M00105-1).
SOX2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-SOX2 Antibody (M00105-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SOX2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-SOX2 Antibody (M00105-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SOX2 Rabbit Monoclonal Antibody
SOX2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SOX2 antibody was positively detected by IHC in:
human cervix carcinoma tissue, human cervical cancer tissue, human tonsilitis tissue, mouse kidney tissue, mouse stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SOX2 antibody was positively detected by IF in:
human cervical cancer tissue, human testis cancer tissue
Recommend Dilution
Application
Dilution
Species
WB 1:1000-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Recommend
Tested
Recommend
Tested
Mouse
Recommend
Tested
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-SOX2 Rabbit Monoclonal Antibody
View all SOX2 Antibodies
View all SOX2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BIO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
34.3 kDa
Cite This Product
Anti-SOX2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00105-1)
Protocols
Immunohistochemistry (IHC) protocol for SOX2 antibody
Immunofluorescence (IF) protocol for SOX2 antibody
Publications
Application:
Species:
KCTD15 Enhances Stem Cell‐Like Properties and Promotes Triple‐Negative Breast Cancer Progression Through KLF4/β‐Catenin Signaling
Published: 2026-02-16
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.20
Species: Human
PMID: 41693649
Carboxymethyl chitosan hydrogel synergistically regulates neural stem cells with ultrasound for the treatment of traumatic brain injury
Published: 2025-10-13
Journal: Materials Today Advances
Application: IF
Impact Factor: 8.00
Species: Mouse
Ginsenoside Rg1 Promotes the Survival, Proliferation, and Differentiation of Senescent Neural Stem Cells Induced by D-galactose
Published: 2025-01-05
Journal: ACTAS ESPANOLAS DE PSIQUIATRIA
Application: IF
Impact Factor: 1.60
Species: Mouse
PMID: 39801411
Review
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Product Promise
We promise Anti-SOX2 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00105-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026