|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SOX2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Transcription factor SOX-2(SOX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SOX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2284)|
|Replacement Item||This antibody may replace the following items: sc-17319|sc-17319-X|sc-17320|sc-17320-X|sc-17351|sc-20088|sc-20088-X|sc-20098|sc-365823|sc-365823-X|sc-365964|sc-365964-X|sc-398254|sc-398254-X|sc-54467|sc-54467-X|sc-54517|sc-54517-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SOX2(171-183aa YSMMQDQLGYPQH), different from the related mouse and rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transcription factor SOX-2|
|Molecular Weight||34310 MW|
|Protein Function||Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). .|
|Sequence Similarities||Contains 1 HMG box DNA-binding domain.|
|Alternative Names||Transcription factor SOX-2;SOX2;|
|Research Areas|||neuroscience|cell type marker|neural stem cell marker| epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| neuroscience|neurology process|neural signal transduction|hmg box|neurogenesis| stem cells|embryonic stem cells|intracellular|germline stem cells|embryonic germ cells| developmental biology|reproduction|germ cell markers|embryogenesis|surface molecules||
Background for Transcription factor SOX-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SOX2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SOX2 Antibody Images
Click the images to enlarge.
IHC(P): Rat Lung Tissue
IHC(P): Mouse Brain Tissue
IHC(P): Human Lung Cancer Tissue
All lanes: Anti-SOX2(PA2284) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,