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Product Info Summary
| SKU: | M00110 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00110
BM4878 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SP1 Rabbit Monoclonal Antibody catalog # M00110. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00110)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOOC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SP1
Reactive Species
M00110 is reactive to SP1 in Human
Observed Molecular Weight
90 kDa
Calculated molecular weight
80.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00110 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SP1 expression in HeLa cell lysate.
Specific Publications For Anti-SP1 Rabbit Monoclonal Antibody (M00110)
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Customer Q&As
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5 Customer Q&As for Anti-SP1 Rabbit Monoclonal Antibody
Question
We are currently using anti-SP1 Rabbit Monoclonal antibody M00110 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The anti-SP1 Rabbit Monoclonal antibody (M00110) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-25
Question
My boss were satisfied with the WB result of your anti-SP1 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus. cytoplasm. note=nuclear location is using this antibody. Is that expected? Could you tell me where is SP1 supposed to be expressed?
S. Jha
Verified customer
Asked: 2019-04-03
Answer
Based on literature, cervix carcinoma erythroleukemia does express SP1. Generally SP1 expresses in nucleus. cytoplasm. note=nuclear location is. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766
Boster Scientific Support
Answered: 2019-04-03
Question
We ordered your anti-SP1 Rabbit Monoclonal antibody for IF on colon a few years ago. I am using human, and We are going to use the antibody for WB next. I am looking for examining colon as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
P. Jackson
Verified customer
Asked: 2017-12-13
Answer
I have checked the website and datasheets of our anti-SP1 Rabbit Monoclonal antibody and it appears that M00110 has been tested on human in both IF and WB. Thus M00110 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-13
Question
I would like using your anti-SP1 Rabbit Monoclonal antibody for positive regulation of angiogenesis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
K. Thomas
Verified customer
Asked: 2016-06-20
Answer
Thanks for your inquiry. This M00110 anti-SP1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-06-20
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-SP1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
O. Jones
Verified customer
Asked: 2013-03-12
Answer
From what I have seen in literature cervix carcinoma does express SP1. From what I have seen in Uniprot.org, SP1 is expressed in nipple, brain testis, cervix carcinoma, colon, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766
Boster Scientific Support
Answered: 2013-03-12
