Anti-Alkaline phosphatase/ALPL Antibody Picoband™

ALPL antibody

Boster Bio Anti-Alkaline phosphatase/ALPL Antibody Picoband™ catalog # A01008. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A01008
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-Alkaline phosphatase/ALPL Antibody Picoband™

View all ALPL Antibodies

SKU/Catalog Number

A01008

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alkaline phosphatase/ALPL Antibody Picoband™ catalog # A01008. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alkaline phosphatase/ALPL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01008)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Alkaline phosphatase/ALPL recombinant protein (Position: K229-H482).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01008 is reactive to ALPL in Human, Mouse, Rat

Applications

A01008 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

57.305kDa

Background of ALPL

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALPL (Source: Uniprot.org, NCBI)

Gene Name

ALPL

Full Name

Alkaline phosphatase, tissue-nonspecific isozyme

Weight

57.305kDa

Superfamily

alkaline phosphatase family

Alternative Names

Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935 ALPL AP-TNAP, APTNAP, HOPS, TNALP, TNAP, TNSALP alkaline phosphatase, biomineralization associated alkaline phosphatase, tissue-nonspecific isozyme|alkaline phosphatase liver/bone/kidney isozyme|alkaline phosphatase, liver/bone/kidney|liver/bone/kidney-type alkaline phosphatase|tissue non-specific alkaline phosphatase|tissue-nonspecific ALP

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALPL, check out the ALPL Infographic

ALPL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALPL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01008 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
A01008 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA

Zhang Y,Yin C,Cheng Y,Huang X,Liu K,Cheng G,Li Z.Electrospinning Nanofiber-Reinforced Aerogels for the Treatment of Bone Defects.Adv Wound Care (New Rochelle).2020 Aug;9(8):441-452.doi:10.1089/wound.2018.0879.Epub 2019 Jun 5.PMID:32857019;PMCID:PMC7382394
Species: Mouse
A01008 usage in article: APP:IF, SAMPLE:MC3T3-E1 CELL, DILUTION:1:200

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Size

Conjugation

Total: $370

SKU:A01008

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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