Product Info Summary
SKU: | A03164 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SPTBN1 Antibody
View all Spectrin beta 2 Antibodies
SKU/Catalog Number
A03164
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SPTBN1 Antibody (Catalog# A03164). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPTBN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03164)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SPTBN1. Immunogen sequence location: 651 - 700
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03164 is reactive to SPTBN1 in Human, Mouse, Rat
Applications
A03164 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
274.609kDa
Background of Spectrin beta 2
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from COLO cells, using SPTBN1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SPTBN1 (Source: Uniprot.org, NCBI)
Gene Name
SPTBN1
Full Name
Spectrin beta chain, non-erythrocytic 1
Weight
274.609kDa
Superfamily
spectrin family
Alternative Names
beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin II; betaSpII; ELF; embryonic liver beta-fodrin; Fodrin beta chain; spectrin beta chain, brain 1; spectrin, beta, non-erythrocytic 1; Spectrin, non-erythroid beta chain 1; SPTB2 SPTBN1 ELF, HEL102, SPTB2, betaSpII spectrin beta, non-erythrocytic 1 spectrin beta chain, non-erythrocytic 1|beta-G spectrin|beta-II spectrin|beta-fodrin|beta-spectrin 2|beta-spectrin II|beta-spectrin non-erythrocytic 1|embryonic liver beta-fodrin|epididymis luminal protein 102|fodrin beta chain|spectrin beta chain, brain 1|spectrin, non-erythroid beta chain 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPTBN1, check out the SPTBN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPTBN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPTBN1 Antibody (A03164)
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No publications found for A03164
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Customer Q&As
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1 Customer Q&As for Anti-SPTBN1 Antibody
Question
We are currently using anti-SPTBN1 antibody A03164 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
The anti-SPTBN1 antibody (A03164) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-27