Click image to see more details
Product Info Summary
| SKU: | P03101 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho HIRA (Thr555) Antibody
SKU/Catalog Number
P03101
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho HIRA (Thr555) Antibody (Catalog# P03101). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho HIRA (Thr555) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03101)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human HIRA around the phosphorylation site of Thr555. Immunogen sequence location: 521-570
Reactive Species
P03101 is reactive to HIRA in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
111.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03101 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho HIRA (Thr555) Antibody (P03101)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho HIRA (Thr555) Antibody
Question
Will P03101 anti-Phospho HIRA (Thr555) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
It shows on the product datasheet, P03101 anti-Phospho HIRA (Thr555) antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-Phospho HIRA (Thr555) antibody P03101 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-Phospho HIRA (Thr555) antibody (P03101) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
Does anti-Phospho HIRA (Thr555) antibody P03101 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to the expression profile of leukemic t-cell, HIRA is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho HIRA (Thr555) antibody P03101 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-01
Question
Is there a BSA free version of anti-Phospho HIRA (Thr555) antibody P03101 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho HIRA (Thr555) antibody P03101 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
Is a blocking peptide available for product anti-Phospho HIRA (Thr555) antibody (P03101)?
D. Krishna
Verified customer
Asked: 2019-04-09
Answer
We do provide the blocking peptide for product anti-Phospho HIRA (Thr555) antibody (P03101). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-04-09
Question
Is this P03101 anti-Phospho HIRA (Thr555) antibody reactive to the isotypes of HIRA?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
The immunogen of P03101 anti-Phospho HIRA (Thr555) antibody is A peptide derived from human HIRA around the phosphorylation site of Thr555. Immunogen sequence location: 521-570. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho HIRA (Thr555) antibody P03101. Let me know if you need anything else.
L. Jackson
Verified customer
Asked: 2016-05-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-31
