Anti-APG7 ATG7 Antibody

ATG7 antibody

Boster Bio Anti-APG7 ATG7 Antibody (Catalog # A00346-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A00346-1
Size: 0.1 mg
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-APG7 ATG7 Antibody

View all ATG7 Antibodies

SKU/Catalog Number

A00346-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-APG7 ATG7 Antibody (Catalog # A00346-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-APG7 ATG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00346-1)

Host

Rabbit

Contents

APG7 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Reactive Species

A00346-1 is reactive to ATG7 in Human, Mouse

Applications

A00346-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

77.96kDa

Background of ATG7

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)

Gene Name

ATG7

Full Name

Ubiquitin-like modifier-activating enzyme ATG7

Weight

77.96kDa

Superfamily

ATG7 family

Alternative Names

APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7 ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATG7, check out the ATG7 Infographic

ATG7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A00346-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-APG7 ATG7 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-APG7 ATG7 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-APG7 ATG7 Antibody

Order DetailsPrice
A00346-1-0.1 mg

0.1 mg

$449

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A00346-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.