Anti-APG7 ATG7 Antibody

Product Name

Anti-APG7 ATG7 Antibody

View all ATG7 Antibodies

SKU/Catalog Number

A00346-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-APG7 ATG7 Antibody (Catalog # A00346-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-APG7 ATG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00346-1)

Host

Rabbit

Contents

APG7 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7.

Cross-reactivity

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Reactive Species

A00346-1 is reactive to ATG7 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

78.0 kDa

Background of ATG7

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00346-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG7

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