Anti-SREBP2/SREBF2 Antibody Picoband®

Western blot analysis of SREBP2/SREBF2 using anti-SREBP2/SREBF2 antibody (A01678-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: rat stomach tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SREBP2/SREBF2 antigen affinity purified polyclonal antibody (Catalog # A01678-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SREBP2/SREBF2 at approximately 68 kDa. The expected band size for SREBP2/SREBF2 is at 124 kDa.

SIRT1 inhibitor EX‐527 increased M1‐like macrophage and lipid accumulation by EGCG in the model cells. (A) Determination of the optimal concentration of EX‐527. n = 3. (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells. n = 3 (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells. n = 3. (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant. n = 6. (E, F) Levels of IL‐4 and IL‐10 in supernatant. n = 6. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05.
Index in PubMed under a CC BY license. PMID: 40801050

TP improved podocyte lipid accumulation and injury in the aging with DKD model rats. (A) Representative TEM images of podocytes in kidney tissues of rats. 20,000×, n = 3. (B) Representative immunofluorescence colocalization images of SYNPO (green fluorescence) and Adipophilin (red fluorescence). 400×, n = 3. (C) The analysis of mean fluorescence density ratio of Adipophilin to SYNPO assay from each group of rats. (D) Representative WB images and quantification of the expression of Nephrin and Podocin. n = 6. (E) Representative WB images and quantification of the expression of SREBP‐1 and SREBP‐2. n = 6. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the TP‐L group, c p < 0.05; compared with the TP‐M group, d p < 0.05.
Index in PubMed under a CC BY license. PMID: 40801050

SIRT1 agonist SRT1720 increased M2‐like macrophage and decreased lipid deposition by EGCG in the model cells. (A) Determination of the optimal concentration of SRT1720 ( n = 3). (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells ( n = 3). (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells ( n = 3). (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant ( n = 6). (E, F) Levels of IL‐4 and IL‐10 in supernatant ( n = 6). Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05.
Index in PubMed under a CC BY license. PMID: 40801050

Flow Cytometry analysis of K562 cells using anti-SREBP2/SREBF2 antibody (A01678-2).
Overlay histogram showing K562 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of RH35 cells using anti-SREBP2/SREBF2 antibody (A01678-2).
Overlay histogram showing RH35 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.