Anti-STAT1 Antibody

Boster Bio Anti-STAT1 Antibody catalog # PA1075. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PA1075
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-STAT1 Antibody

See all STAT1 products

SKU/Catalog Number

PA1075

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAT1 Antibody catalog # PA1075. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1075)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human STAT1 (364-378aa FDKDVNERNTVKGFR), different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1075 is reactive to STAT1 in Human, Mouse, Rat

Applications

PA1075 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For STAT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P42224

Gene ID

6772

Gene Name

STAT1

Full Name

Signal transducer and activator of transcription 1-alpha/beta

Weight

87.335kDa

Superfamily

transcription factor STAT family

Alternative Names

CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STAT1, check out the STAT1 Infographic

STAT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for STAT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1075 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway

Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Song Cg, Yang X, Min Lq, Liu Cx, Zhao Cs. Neuro Endocrinol Lett. 2014;35(1):68-72. The Effect Of Procyanidin On Expression Of Stat1 In Type 2 Diabetes Mellitus Sd Rats With Focal Cerebral Ischemia.

Have you used Anti-STAT1 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-STAT1 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-STAT1 Antibody

Question

I was wanting to use to test anti-STAT1 antibody PA1075 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-STAT1 antibody PA1075 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Parker

Verified customer

Asked: 2020-04-28

Answer

The products we sell, including anti-STAT1 antibody PA1075, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-28

Question

We want using your anti-STAT1 antibody for response to peptide hormone studies. Has this antibody been tested with western blotting on thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

Thank you for your inquiry. This PA1075 anti-STAT1 antibody is tested on hela cell lysate, mouse ovary tissue, heart tissue, tissue lysate, liver tissue, brain tissue, kidney tissue, spleen tissue, thymus tissue, lung tissue, intestine tissue, rat thymus tissue, testis tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-10

Question

Is this PA1075 anti-STAT1 antibody reactive to the isotypes of STAT1?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The immunogen of PA1075 anti-STAT1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human STAT1(364-378aa FDKDVNERNTVKGFR), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-31

Question

Would PA1075 anti-STAT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

You can see on the product datasheet, PA1075 anti-STAT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-19

Question

I have a question about product PA1075, anti-STAT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1075 anti-STAT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-09

Question

Is there a BSA free version of anti-STAT1 antibody PA1075 available?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT1 antibody PA1075 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-25

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT1 antibody PA1075. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-19

Question

Our team were happy with the WB result of your anti-STAT1 antibody. However we have observed positive staining in cervix carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

According to literature, cervix carcinoma does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-09-06

Question

I was wanting to use your anti-STAT1 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

As indicated on the product datasheet, PA1075 anti-STAT1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-30

Question

Would anti-STAT1 antibody PA1075 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

According to the expression profile of cervix carcinoma, STAT1 is highly expressed in cervix carcinoma. So, it is likely that anti-STAT1 antibody PA1075 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-07-01

Question

I see that the anti-STAT1 antibody PA1075 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-21

Question

Is a blocking peptide available for product anti-STAT1 antibody (PA1075)?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

We do provide the blocking peptide for product anti-STAT1 antibody (PA1075). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-12

Question

We are currently using anti-STAT1 antibody PA1075 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-29

Answer

The anti-STAT1 antibody (PA1075) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-29

Question

We have observed staining in human epithelium of bronchus. Do you have any suggestions? Is anti-STAT1 antibody supposed to stain epithelium of bronchus positively?

C. Anderson

Verified customer

Asked: 2017-08-29

Answer

From literature epithelium of bronchus does express STAT1. From Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-08-29

Question

Would anti-STAT1 antibody PA1075 work on pig WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

Our lab technicians have not tested anti-STAT1 antibody PA1075 on pig. You can run a BLAST between pig and the immunogen sequence of anti-STAT1 antibody PA1075 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT1 antibody PA1075. Let me know if you need anything else.

R. Gonzalez

Verified customer

Asked: 2016-04-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-04-01

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PA1075
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.