Product Info Summary
SKU: | A02219-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IL-3RB/Csf2rb Antibody Picoband™
View all Common beta Chain Antibodies
SKU/Catalog Number
A02219-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-3RB/Csf2rb Antibody Picoband™ catalog # A02219-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-3RB/Csf2rb Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02219-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouseIL-3RB/Csf2rb recombinant protein (Position: A28-Q891).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02219-3 is reactive to CSF2RB in Mouse, Rat
Applications
A02219-3 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
97.336kDa
Background of Common beta Chain
Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat
Flow Cytometry, 1-3μg/1x106 cells, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CSF2RB using anti-CSF2RB antibody (A02219-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF2RB antigen affinity purified polyclonal antibody (Catalog # A02219-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF2RB at approximately 120KD. The expected band size for CSF2RB is at 120KD.
Click image to see more details
Figure 2. IHC analysis of CSF2RB using anti-CSF2RB antibody (A02219-3).
CSF2RB was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSF2RB Antibody (A02219-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-CSF2RB antibody (A02219-3).
Overlay histogram showing ANA-1 cells stained with A02219-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSF2RB Antibody (A02219-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Flow Cytometry analysis of NRK cells using anti-CSF2RB antibody (A02219-3).
Overlay histogram showing NRK cells stained with A02219-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSF2RB Antibody (A02219-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CSF2RB (Source: Uniprot.org, NCBI)
Gene Name
CSF2RB
Full Name
Cytokine receptor common subunit beta
Weight
97.336kDa
Superfamily
type I cytokine receptor family
Alternative Names
beta, low-affinity; CD131 antigen; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage); Common beta Chain; CSF2RB; cytokine receptor common subunit beta; GM-CSF R beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 R beta; IL3RB; IL-5 R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity) CSF2RB CD131, CDw131, IL3RB, IL5RB, SMDP5, betaGMR colony stimulating factor 2 receptor subunit beta cytokine receptor common subunit beta|GM-CSF/IL-3/IL-5 receptor common beta subunit|GM-CSF/IL-3/IL-5 receptor common beta-chain|beta common cytokine receptor|beta-GM-CSF receptor|colony stimulating factor 2 receptor beta common subunit|colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)|colony-stimulating factor-2 receptor, beta, low-affinity|interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSF2RB, check out the CSF2RB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSF2RB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-3RB/Csf2rb Antibody Picoband™ (A02219-3)
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