Anti-survivin/BIRC5 Antibody

Survivin antibody

Boster Bio Anti-survivin/BIRC5 Antibody catalog # PA1474. Tested in WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PA1474
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-survivin/BIRC5 Antibody

View all Survivin Antibodies

SKU/Catalog Number

PA1474

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-survivin/BIRC5 Antibody catalog # PA1474. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-survivin/BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1474)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human survivin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1474 is reactive to BIRC5 in Human

Applications

PA1474 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

16.389kDa

Background of Survivin

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BIRC5 (Source: Uniprot.org, NCBI)

Gene Name

BIRC5

Full Name

Baculoviral IAP repeat-containing protein 5

Weight

16.389kDa

Superfamily

IAP family

Alternative Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BIRC5, check out the BIRC5 Infographic

BIRC5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BIRC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1474 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Increased survivin expression and its association with clinical parameters of congenital choledochal cysts

Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Shan Sj, Zhang N, Geng Sl, Zhou Z, Chen X, Nie T, Guo Z, Li C, Liu Q, Guo Y, Wei H, Chen Hd. J Cutan Pathol. 2010 Jun;37(6):635-40. Doi: 10.1111/J.1600-0560.2009.01437.X. Epub 2009 Sep 24. Expression Of Survivin And Human Telomerase Reverse Transc...

Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...

Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.

Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...

Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...

Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

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4 Customer Q&As for Anti-survivin/BIRC5 Antibody

Question

I am interested in using your anti-survivin/BIRC5 antibody for negative regulation of transcription studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

Thank you for your inquiry. This PA1474 anti-survivin/BIRC5 antibody is tested on hela cell lysate, jurkat cell lysate, cem cell lysate, colo320 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-12

Question

Our team were happy with the WB result of your anti-survivin/BIRC5 antibody. However we have seen positive staining in vagina cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

From literature, vagina does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2020-02-28

Question

We have been able to see staining in human myeloid leukemia cell. Do you have any suggestions? Is anti-survivin/BIRC5 antibody supposed to stain myeloid leukemia cell positively?

Verified Customer

Verified customer

Asked: 2018-12-11

Answer

According to literature myeloid leukemia cell does express BIRC5. According to Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2018-12-11

Question

We are currently using anti-survivin/BIRC5 antibody PA1474 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

A. Baker

Verified customer

Asked: 2018-07-24

Answer

The anti-survivin/BIRC5 antibody (PA1474) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-24

Order DetailsPrice
PA1474

100μg

$370
PA1474-10ug

10μg sample (liquid)

$99
PA1474-Biotin

100 μg Biotin conjugated

$570
PA1474-Cy3

100 μg Cy3 conjugated

$570
PA1474-Dylight488

100 μg Dylight488 conjugated

$570
PA1474-Dylight550

100 μg Dylight550 conjugated

$570
PA1474-Dylight594

100 μg Dylight594 conjugated

$570
PA1474-FITC

100 μg FITC conjugated

$570
PA1474-HRP

100 μg HRP conjugated

$570
PA1474-APC

100 μg APC conjugated

$670
PA1474-PE

100 μg PE conjugated

$670
PA1474-carrier-free

Carrier Free

$370

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