Rabbit IgG polyclonal antibody for Baculoviral IAP repeat-containing protein 5(BIRC5) detection. Tested with WB in Human. Cited in 12 publication(s).
Anti-survivin/BIRC5 Antibody Info At A Glance
|Product Name||Anti-survivin/BIRC5 Antibody
See all Survivin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for 536 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. 536 information: Molecular Weight: 16389 MW; Subcellular Localization: Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export; Tissue Specificity: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.|
|Cite This Product||Anti-survivin/BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1474)|
|Similar Products From
|Anti-survivin/BIRC5 Antibody may replace the following items: sc 163274|sc 163273.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human survivin(121-142aa KKEFEETAKKVRRAIEQLAAMD).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For BIRC5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||332|
|Species Of This Entry||Human|
|Protein Name||Baculoviral IAP repeat-containing protein 5|
|Alternative Names||Survivin|API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Acetylation; Ubiquitination; Dephosphorylation; Reduction; Deacetylation; Phosphorothioation; Prenylation; Demethylation; Amidation; Biotinylation; Nitrosylation; Citrullination; Farnesylation; Geranylgeranylation; Myristoylation|
|Subcellular Localization||Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, l|
|Gene Location||17q25.3, on Chromasome 17, gene sequence: NC_000017.11 (78214253..78225635)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For BIRC5 (Source: Uniprot.org, NCBI)
|Protein Function||Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).|
|Tissue Specificity||Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).|
|Interacting Proteins||AURKA; AURKB; AURKC; BECN1; CASP9; CDCA8; CDK1; DIABLO; GAS2L3; INCENP; RAN; XPO1|
|Research Areas||Adaptive Immunity; Angiogenesis; Apoptosis; Astrocyte Markers; Cancer; Growth and Development; Innate Immunity; Mitotic Regulators; Phospho-Specific|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Paraganglionic Neoplasm; Neoplasm Metastasis; Malignant Neoplasm Of Breast; Mammary Neoplasms; Lung Neoplasms; Malignant Neoplasm Of Lung; Cell Transformation, Neoplastic; Malignant Squamous Cell Neoplasm; Adenocarcinoma; Leukemia; Liver Carcinoma; Carcinogenesis; Liver Neoplasms; Colorectal Cancer; Bladder Neoplasm; Non-small Cell Lung Carcinoma|
|Related Pathways||Cell Cycle; Cell Death; Cell Proliferation; Cell Growth; Mitosis; Cell Division; Localization; Induction Of Apoptosis; Angiogenesis; Rna Interference; Cell Cycle Arrest; Pathogenesis; Drug Resistance; Cytokinesis; Programmed Cell Death; Reverse Transcription; Oncogenesis; Gene Silencing; Sensitization; Chromosome Segregation|
|Background||Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis(IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.|
|Scientific References||PMID: 9256286 by Ambrosini G., et al. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.|
PMID: 10626797 by Mahotka C., et al. Survivin-deltaEx3 and survivin-2B: two novel splice variants of the apoptosis inhibitor survivin with different antiapoptotic properties.
Our Boster Quality Guarantee for Anti-survivin/BIRC5 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: CEM Cell Lysate
Lane 4: COLO320 Cell Lysate
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Total number of citations: 12
|Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...||PubMed ID 25333250|
|Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.||PubMed ID 24760075|
|Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...||PubMed ID 23428654|
|Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...||PubMed ID 22773975|
|Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.||PubMed ID 24519067|
|Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...||PubMed ID 23611778|
|Shan Sj, Zhang N, Geng Sl, Zhou Z, Chen X, Nie T, Guo Z, Li C, Liu Q, Guo Y, Wei H, Chen Hd. J Cutan Pathol. 2010 Jun;37(6):635-40. Doi: 10.1111/J.1600-0560.2009.01437.X. Epub 2009 Sep 24. Expression Of Survivin And Human Telomerase Reverse Transc...||PubMed ID 19788441|
|Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing||PubMed ID 27123101|
|Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance||PubMed ID 26823716|
|Reactive oxygen species mediate isoalantolactone-induced apoptosis in human prostate cancer cells||PubMed ID 23921797|
|Increased survivin expression and its association with clinical parameters of congenital choledochal cysts||PubMed ID 27420945|
|Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by %u2026||PubMed ID 29038528|
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