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Product Info Summary
| SKU: | PA1474 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-survivin/BIRC5 Antibody Picoband®
SKU/Catalog Number
PA1474
BA4055-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-survivin/BIRC5 Antibody catalog # PA1474. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-survivin/BIRC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1474)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human survivin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1474 is reactive to BIRC5 in Human
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.4 kDa
Background of BIRC5
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1474 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human PC-3 whole cell, human U20S whole cell, human 293T whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Specific Publications For Anti-survivin/BIRC5 Antibody Picoband® (PA1474)
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Customer Q&As
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4 Customer Q&As for Anti-survivin/BIRC5 Antibody Picoband®
Question
I am interested in using your anti-survivin/BIRC5 antibody for negative regulation of transcription studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Thank you for your inquiry. This PA1474 anti-survivin/BIRC5 antibody is tested on hela cell lysate, jurkat cell lysate, cem cell lysate, colo320 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-12
Question
Our team were happy with the WB result of your anti-survivin/BIRC5 antibody. However we have seen positive staining in vagina cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
From literature, vagina does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2020-02-28
Question
We have been able to see staining in human myeloid leukemia cell. Do you have any suggestions? Is anti-survivin/BIRC5 antibody supposed to stain myeloid leukemia cell positively?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
According to literature myeloid leukemia cell does express BIRC5. According to Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2018-12-11
Question
We are currently using anti-survivin/BIRC5 antibody PA1474 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
A. Baker
Verified customer
Asked: 2018-07-24
Answer
The anti-survivin/BIRC5 antibody (PA1474) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-24
