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- Table of Contents
BIRC5 antibody (RP1026) performs well in ELISA, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026).
Survivin/BIRC5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/BIRC5 Antibody (RP1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Survivin/BIRC5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/BIRC5 Antibody (RP1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-survivin/BIRC5 Antibody Picoband®
BIRC5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC, WB |
Tested Applications
BIRC5 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human 293T whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BIRC5 antibody was positively detected by IHC in:
human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-survivin/BIRC5 Antibody Picoband®
View all BIRC5 Antibodies
View all BIRC5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Survivin recombinant protein (Position: M1-D142).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated Molecular Weight
16.4 kDa
Background of BIRC5
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Cite This Product
Anti-survivin/BIRC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1026)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
92%
High Homology
Cow
91%
High Homology
Sheep
90%
High Homology
Rat
85%
Moderate Homology
Mouse
83%
Moderate Homology
Zebrafish
50%
Low homology
Rabbit
42%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for BIRC5 antibody
Immunohistochemistry (IHC) protocol for BIRC5 antibody
Publications
Application:
Species:
Isolation of an “Early” Transit Amplifying Keratinocyte Population in Human Epidermis: A Role for the Low Affinity Neurotrophin Receptor CD271
Published: 2022-08-29
Journal: STEM CELLS
Application: WB
Impact Factor: 5.85
Species: Human
PMID: 36037263
Effect of 5-aminolevulinic acid photodynamic therapy on the expression of apoptosis inhibitors Bcl-2 and Survivin in keratinocytes of condyloma acuminatum
Published: 2019-07-23
Journal: Photodiagnosis and Photodynamic Therapy
Application: IHC
Impact Factor: 2.59
Species: Human
PMID: 31349100
Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through suppression of Mcl-1
Published: 2013-04-20
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.41
Species: Human
PMID: 23611778
Induction of Apoptosis by Costunolide in Bladder Cancer Cells is Mediated through ROS Generation and Mitochondrial Dysfunction
Published: 2013-01-24
Journal: MOLECULES
Application: WB
Impact Factor: 2.43
Species: Human
PMID: 23348995
Downregulation of survivin and activation of caspase-3 through the PI3K/Akt pathway in ursolic acid-induced HepG2 cell apoptosis
Published: 2009-04-01
Journal: ANTI-CANCER DRUGS
Application: WB
Impact Factor: 2.36
Species: Human
PMID: 19174695
Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China
Published: 2008-06-20
Journal: Journal of Translational Medicine
Application: IHC
Impact Factor: 2.94
Species: Human
PMID: 18570662
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Product Promise
We promise Anti-survivin/BIRC5 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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RP1026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026