Anti-survivin/BIRC5 Antibody

Boster Bio Anti-survivin/BIRC5 Antibody catalog # RP1026. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Cited in 9 publication(s).

Product Info Summary

SKU: RP1026
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-survivin/BIRC5 Antibody

See all Survivin products

SKU/Catalog Number

RP1026

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-survivin/BIRC5 Antibody catalog # RP1026. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-survivin/BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1026)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human Survivin recombinant protein (Position: M1-D142).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1026 is reactive to BIRC5 in Human

Applications

RP1026 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BIRC5 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15392

Gene ID

332

Gene Name

BIRC5

Full Name

Baculoviral IAP repeat-containing protein 5

Weight

16.389kDa

Superfamily

IAP family

Alternative Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BIRC5, check out the BIRC5 Infographic

BIRC5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BIRC5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1026 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Increased survivin expression and its association with clinical parameters of congenital choledochal cysts

Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Radiosensitivity of human colon cancer cell enhanced by immunoliposomal docetaxel

Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...

Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.

Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...

Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

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18 Customer Q&As for Anti-survivin/BIRC5 Antibody

Question

We have been able to see staining in human myeloid leukemia cell. What should we do? Is anti-survivin/BIRC5 antibody supposed to stain myeloid leukemia cell positively?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

Based on literature myeloid leukemia cell does express BIRC5. Based on Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2020-04-27

Question

Would RP1026 anti-survivin/BIRC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

As indicated on the product datasheet, RP1026 anti-survivin/BIRC5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-17

Question

I was wanting to use your anti-survivin/BIRC5 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

It shows on the product datasheet, RP1026 anti-survivin/BIRC5 antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-12

Question

Does anti-survivin/BIRC5 antibody RP1026 work for WB with lung?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

According to the expression profile of lung, BIRC5 is highly expressed in lung. So, it is likely that anti-survivin/BIRC5 antibody RP1026 will work for WB with lung.

Boster Scientific Support

Answered: 2020-03-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-survivin/BIRC5 antibody RP1026. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-02

Question

We need using your anti-survivin/BIRC5 antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on human hek293 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

I appreciate your inquiry. This RP1026 anti-survivin/BIRC5 antibody is tested on human hek293 whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-survivin/BIRC5 antibody RP1026 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-22

Answer

The anti-survivin/BIRC5 antibody (RP1026) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-22

Question

Please see the WB image, lot number and protocol we used for lung using anti-survivin/BIRC5 antibody RP1026. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-08-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-07

Question

Does anti-survivin/BIRC5 antibody RP1026 work on bovine IHC with mammary gland muscle?

Verified Customer

Verified customer

Asked: 2018-04-09

Answer

Our lab technicians have not validated anti-survivin/BIRC5 antibody RP1026 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-survivin/BIRC5 antibody RP1026 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine mammary gland muscle in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-09

Question

I would like to test anti-survivin/BIRC5 antibody RP1026 on human lung for research purposes, then I may be interested in using anti-survivin/BIRC5 antibody RP1026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-12-27

Answer

The products we sell, including anti-survivin/BIRC5 antibody RP1026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-12-27

Question

Is a blocking peptide available for product anti-survivin/BIRC5 antibody (RP1026)?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

We do provide the blocking peptide for product anti-survivin/BIRC5 antibody (RP1026). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-19

Question

Can you help my question with product RP1026, anti-survivin/BIRC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-08-30

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1026 anti-survivin/BIRC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-08-30

Question

Is this RP1026 anti-survivin/BIRC5 antibody reactive to the isotypes of BIRC5?

K. Walker

Verified customer

Asked: 2016-09-01

Answer

The immunogen of RP1026 anti-survivin/BIRC5 antibody is E. coli-derived human Survivin recombinant protein(Position: M1-D142). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-09-01

Question

Do you have a BSA free version of anti-survivin/BIRC5 antibody RP1026 available?

P. Anderson

Verified customer

Asked: 2016-04-08

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-survivin/BIRC5 antibody RP1026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-04-08

Question

Our lab used your anti-survivin/BIRC5 antibody for IHC on myeloid leukemia cell in a previous project. I am using human, and We intend to use the antibody for WB next. My lab would like examining myeloid leukemia cell as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

K. Jones

Verified customer

Asked: 2015-01-02

Answer

I looked at the website and datasheets of our anti-survivin/BIRC5 antibody and I see that RP1026 has been tested on human in both IHC and WB. Thus RP1026 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-01-02

Question

By saying the recombinant human survivin protein, do you mean that some specific tag was used which increased the overall MW weight to 34kd? We observed 34kd band size with the anti-survivin antibody with the mouse liver lysate, the reason we do not currently know. We surmise it as a dimeric form of the survivin. Thanks for your input.

Verified Customer

Verified customer

Asked: 2014-12-09

Answer

The 34kd of the recombinant protein is the dimeric form of the survivin. Please see the updated image wiht bands at 16 kD on the product page. https://www.bosterbio.com/anti-human-survivin-antibody-rp1026-boster.html?options=cart

Boster Scientific Support

Answered: 2014-12-09

Question

Our team were content with the WB result of your anti-survivin/BIRC5 antibody. However we have been able to see positive staining in mammary gland muscle cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?

M. Mitchell

Verified customer

Asked: 2014-01-15

Answer

Based on literature, mammary gland muscle does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2014-01-15

Question

I see that the anti-survivin/BIRC5 antibody RP1026 works with WB, what is the protocol used to produce the result images on the product page?

J. Taylor

Verified customer

Asked: 2013-11-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-11-01

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RP1026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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