Anti-Survivin/BIRC5 Antibody

Boster Bio Anti-Survivin/BIRC5 Antibody catalog # RP1052. Tested in WB applications. This antibody reacts with Mouse. Cited in 9 publication(s).

Product Info Summary

SKU: RP1052
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Survivin/BIRC5 Antibody

See all Survivin products

SKU/Catalog Number

RP1052

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Survivin/BIRC5 Antibody catalog # RP1052. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Survivin/BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1052)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1052 is reactive to Birc5 in Mouse

Applications

RP1052 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Birc5 (Source: Uniprot.Org, NCBI)

Uniprot ID

O70201

Gene ID

11799

Gene Name

Birc5

Full Name

Baculoviral IAP repeat-containing protein 5

Weight

16.298kDa

Superfamily

IAP family

Alternative Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin Birc5|A, AAC-11, Api4, T, TIAP, s, survivin40|baculoviral IAP repeat-containing 5|baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Birc5, check out the Birc5 Infographic

Birc5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Birc5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1052 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Shan Sj, Zhang N, Geng Sl, Zhou Z, Chen X, Nie T, Guo Z, Li C, Liu Q, Guo Y, Wei H, Chen Hd. J Cutan Pathol. 2010 Jun;37(6):635-40. Doi: 10.1111/J.1600-0560.2009.01437.X. Epub 2009 Sep 24. Expression Of Survivin And Human Telomerase Reverse Transc...

Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...

Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.

Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...

Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...

Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

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15 Customer Q&As for Anti-Survivin/BIRC5 Antibody

Question

Our team were content with the WB result of your anti-Survivin/BIRC5 antibody. However we have seen positive staining in lung cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

Based on literature, lung does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2020-05-04

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody RP1052. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-17

Question

I see that the anti-Survivin/BIRC5 antibody RP1052 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-29

Question

Is a blocking peptide available for product anti-Survivin/BIRC5 antibody (RP1052)?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

We do provide the blocking peptide for product anti-Survivin/BIRC5 antibody (RP1052). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-01

Question

We are interested in to test anti-Survivin/BIRC5 antibody RP1052 on mouse myeloid leukemia cell for research purposes, then I may be interested in using anti-Survivin/BIRC5 antibody RP1052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

The products we sell, including anti-Survivin/BIRC5 antibody RP1052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-20

Question

I have a question about product RP1052, anti-Survivin/BIRC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1052 anti-Survivin/BIRC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-03

Question

Is this RP1052 anti-Survivin/BIRC5 antibody reactive to the isotypes of BIRC5?

T. Taylor

Verified customer

Asked: 2019-02-19

Answer

The immunogen of RP1052 anti-Survivin/BIRC5 antibody is E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-19

Question

Will RP1052 anti-Survivin/BIRC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

As indicated on the product datasheet, RP1052 anti-Survivin/BIRC5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-21

Question

I was wanting to use your anti-Survivin/BIRC5 antibody for WB for mouse myeloid leukemia cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse myeloid leukemia cell identification?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

You can see on the product datasheet, RP1052 anti-Survivin/BIRC5 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-15

Question

Is there a BSA free version of anti-Survivin/BIRC5 antibody RP1052 available?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Survivin/BIRC5 antibody RP1052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-02-05

Question

We are currently using anti-Survivin/BIRC5 antibody RP1052 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?

D. Jones

Verified customer

Asked: 2016-10-04

Answer

The anti-Survivin/BIRC5 antibody (RP1052) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-10-04

Question

We have observed staining in mouse mammary cancer. What should we do? Is anti-Survivin/BIRC5 antibody supposed to stain mammary cancer positively?

T. Li

Verified customer

Asked: 2016-08-04

Answer

According to literature mammary cancer does express BIRC5. According to Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722

Boster Scientific Support

Answered: 2016-08-04

Question

Does anti-Survivin/BIRC5 antibody RP1052 work for WB with myeloid leukemia cell?

D. Gonzalez

Verified customer

Asked: 2016-07-20

Answer

According to the expression profile of myeloid leukemia cell, BIRC5 is highly expressed in myeloid leukemia cell. So, it is likely that anti-Survivin/BIRC5 antibody RP1052 will work for WB with myeloid leukemia cell.

Boster Scientific Support

Answered: 2016-07-20

Question

I have attached the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody RP1052. Please let me know if you require anything else.

T. Carter

Verified customer

Asked: 2013-09-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-02

Question

My lab would like using your anti-Survivin/BIRC5 antibody for regulation of insulin secretion involved in cellular response to glucose stimulus studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

W. Wu

Verified customer

Asked: 2013-06-06

Answer

We appreciate your inquiry. This RP1052 anti-Survivin/BIRC5 antibody is validated on mouse liver tissue, kidney tissue, tissue lysate, testis tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-06-06

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RP1052
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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