Product Info Summary
SKU: | RP1052 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Survivin/BIRC5 Antibody
SKU/Catalog Number
RP1052
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Survivin/BIRC5 Antibody catalog # RP1052. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Survivin/BIRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1052)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1052 is reactive to BIRC5 in Mouse
Applications
RP1052 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.389kDa
Background of Survivin
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mous
Flow Cytometry, 1-3 μg/1x106 cells, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (RP1052).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse SP2/0 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates,
Lane 4: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # RP1052) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
Click image to see more details
Figure 2. IHC analysis of Survivin using anti-Survivin antibody (RP1052).
Survivin was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (RP1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Survivin using anti-Survivin antibody (RP1052).
Survivin was detected in a paraffin-embedded section of mouse lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (RP1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of Neuro2a cells using anti-Survivin antibody (RP1052).
Overlay histogram showing Neuro2a cells stained with RP1052 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Survivin Antibody (RP1052, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BIRC5 (Source: Uniprot.org, NCBI)
Gene Name
BIRC5
Full Name
Baculoviral IAP repeat-containing protein 5
Weight
16.389kDa
Superfamily
IAP family
Alternative Names
API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BIRC5, check out the BIRC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BIRC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Survivin/BIRC5 Antibody (RP1052)
Hello CJ!
RP1052 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of livin, survivin and caspase-3 in prostatic cancer and their clinical significance
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
Shan Sj, Zhang N, Geng Sl, Zhou Z, Chen X, Nie T, Guo Z, Li C, Liu Q, Guo Y, Wei H, Chen Hd. J Cutan Pathol. 2010 Jun;37(6):635-40. Doi: 10.1111/J.1600-0560.2009.01437.X. Epub 2009 Sep 24. Expression Of Survivin And Human Telomerase Reverse Transc...
Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...
Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...
Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
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Customer Q&As
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15 Customer Q&As for Anti-Survivin/BIRC5 Antibody
Question
Our team were content with the WB result of your anti-Survivin/BIRC5 antibody. However we have seen positive staining in lung cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Based on literature, lung does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2020-05-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody RP1052. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-17
Question
I see that the anti-Survivin/BIRC5 antibody RP1052 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-29
Question
Is a blocking peptide available for product anti-Survivin/BIRC5 antibody (RP1052)?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
We do provide the blocking peptide for product anti-Survivin/BIRC5 antibody (RP1052). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-01
Question
We are interested in to test anti-Survivin/BIRC5 antibody RP1052 on mouse myeloid leukemia cell for research purposes, then I may be interested in using anti-Survivin/BIRC5 antibody RP1052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The products we sell, including anti-Survivin/BIRC5 antibody RP1052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-20
Question
I have a question about product RP1052, anti-Survivin/BIRC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1052 anti-Survivin/BIRC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this RP1052 anti-Survivin/BIRC5 antibody reactive to the isotypes of BIRC5?
T. Taylor
Verified customer
Asked: 2019-02-19
Answer
The immunogen of RP1052 anti-Survivin/BIRC5 antibody is E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-19
Question
Will RP1052 anti-Survivin/BIRC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
As indicated on the product datasheet, RP1052 anti-Survivin/BIRC5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-21
Question
I was wanting to use your anti-Survivin/BIRC5 antibody for WB for mouse myeloid leukemia cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse myeloid leukemia cell identification?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
You can see on the product datasheet, RP1052 anti-Survivin/BIRC5 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-15
Question
Is there a BSA free version of anti-Survivin/BIRC5 antibody RP1052 available?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Survivin/BIRC5 antibody RP1052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-05
Question
We are currently using anti-Survivin/BIRC5 antibody RP1052 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?
D. Jones
Verified customer
Asked: 2016-10-04
Answer
The anti-Survivin/BIRC5 antibody (RP1052) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-10-04
Question
We have observed staining in mouse mammary cancer. What should we do? Is anti-Survivin/BIRC5 antibody supposed to stain mammary cancer positively?
T. Li
Verified customer
Asked: 2016-08-04
Answer
According to literature mammary cancer does express BIRC5. According to Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2016-08-04
Question
Does anti-Survivin/BIRC5 antibody RP1052 work for WB with myeloid leukemia cell?
D. Gonzalez
Verified customer
Asked: 2016-07-20
Answer
According to the expression profile of myeloid leukemia cell, BIRC5 is highly expressed in myeloid leukemia cell. So, it is likely that anti-Survivin/BIRC5 antibody RP1052 will work for WB with myeloid leukemia cell.
Boster Scientific Support
Answered: 2016-07-20
Question
I have attached the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin/BIRC5 antibody RP1052. Please let me know if you require anything else.
T. Carter
Verified customer
Asked: 2013-09-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-02
Question
My lab would like using your anti-Survivin/BIRC5 antibody for regulation of insulin secretion involved in cellular response to glucose stimulus studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
W. Wu
Verified customer
Asked: 2013-06-06
Answer
We appreciate your inquiry. This RP1052 anti-Survivin/BIRC5 antibody is validated on mouse liver tissue, kidney tissue, tissue lysate, testis tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-06-06