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Product Info Summary
| SKU: | A00361 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-TEL ETV6 Antibody
SKU/Catalog Number
A00361
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TEL ETV6 Antibody catalog # A00361. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TEL ETV6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00361)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ETV6. AA range:371-420
Reactive Species
A00361 is reactive to ETV6 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
53.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00361 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using TEL Polyclonal Antibody
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Western blot analysis of lysates from HeLa, HepG2, and Jurkat cells, using ETV6 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of HeLa cells, using ETV6 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TEL ETV6 Antibody (A00361)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TEL ETV6 Antibody
Question
Can you help my question with product A00361, anti-TEL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00361 anti-TEL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-18
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-TEL antibody A00361. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Is a blocking peptide available for product anti-TEL antibody (A00361)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-TEL antibody (A00361). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
I was wanting to use your anti-TEL antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
It shows on the product datasheet, A00361 anti-TEL antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
Is this A00361 anti-TEL antibody reactive to the isotypes of ETV6?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
The immunogen of A00361 anti-TEL antibody is Synthesized peptide derived from the C-terminal region of human TEL. at AA rangle: 340-420. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-13
Question
We are interested in to test anti-TEL antibody A00361 on rat cervix carcinoma for research purposes, then I may be interested in using anti-TEL antibody A00361 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
The products we sell, including anti-TEL antibody A00361, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-18
Question
Does anti-TEL antibody A00361 work for WB with cervix carcinoma?
K. Anderson
Verified customer
Asked: 2017-04-25
Answer
According to the expression profile of cervix carcinoma, ETV6 is highly expressed in cervix carcinoma. So, it is likely that anti-TEL antibody A00361 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2017-04-25
