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Product Info Summary
| SKU: | M05546-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379
SKU/Catalog Number
M05546-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (Catalog # M05546-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05546-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM379
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to human Terminal deoxynucleotidyl transferase (TdT)
Cross-reactivity
This antibody reacts to human Terminal deoxynucleotidyl transferase (TdT)
Reactive Species
M05546-1 is reactive to DNTT in Human
Calculated molecular weight
58.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05546-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of Jurkat cells lysate using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:2000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thymus tissue section using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:200 dilution.
Specific Publications For Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (M05546-1)
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5 Customer Q&As for Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379
Question
My colleagues were well pleased with the WB result of your anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
From what I have seen in literature, thymus does express DNTT. Generally DNTT expresses in nucleus. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2020-05-08
Question
We have tried in the past anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
I took a look at the website and datasheets of our anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 M05546-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 (M05546-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
We have been able to see staining in human bone marrow. Any tips? Is anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 supposed to stain bone marrow positively?
R. Li
Verified customer
Asked: 2015-11-18
Answer
From literature bone marrow does express DNTT. From Uniprot.org, DNTT is expressed in bone marrow, thymus, testis, among other tissues. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2015-11-18
Question
I am looking for using your anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 for base-excision repair studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
G. Roberts
Verified customer
Asked: 2015-11-04
Answer
We appreciate your inquiry. This M05546-1 anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 is tested on jurkat cells, human thymus tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-11-04
