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Product Info Summary
| SKU: | M00359 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Canine, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Thyroglobulin TG Monoclonal Antibody
SKU/Catalog Number
M00359
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Thyroglobulin TG Monoclonal Antibody (Catalog # M00359). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Thyroglobulin TG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00359)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 2H11
Isotype
IgG1
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00359 is reactive to TG in Canine, Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
304.8 kDa
Background of TG
Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid and secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00359 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:100,000
IHC: 1:50 - 1:200
WB: 1:50 - 1:200
Anti-Thyroglobulin antibody has been tested by western blot and is suitable for the detection of thyroglobulin in ELISA, immunohistochemistry, and immunoprecipitation. For immunohistochemistry, both frozen sections and formalin fixed, paraffin-embedded tissue sections can be used without epitope retrieval or enzyme digestion. This antibody is specific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmic localization. Thyroid tissue can be used as a positive control.
Validation Images & Assay Conditions
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Western blot using Boster Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Specific Publications For Anti-Thyroglobulin TG Monoclonal Antibody (M00359)
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Customer Q&As
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4 Customer Q&As for Anti-Thyroglobulin TG Monoclonal Antibody
Question
Is there a BSA free version of anti-Thyroglobulin Monoclonal antibody M00359 available?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Thyroglobulin Monoclonal antibody M00359 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-13
Question
Would M00359 anti-Thyroglobulin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
It shows on the product datasheet, M00359 anti-Thyroglobulin Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-24
Question
My question regarding product M00359, anti-Thyroglobulin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00359 anti-Thyroglobulin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Thyroglobulin Monoclonal antibody M00359. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-11
