Anti-Insig1 Antibody Picoband™

Product Name

Anti-Insig1 Antibody Picoband™

View all INSIG-1 Antibodies

SKU/Catalog Number

A02026-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Insig1 Antibody Picoband™ catalog # A02026-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Insig1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02026-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of rat Insig1, which shares 71.4% and 92.9% amino acid (aa) sequence identity with human and mouse Insig1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02026-1 is reactive to INSIG1 in Mouse, Rat

Applications

A02026-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

29.987kDa

Background of INSIG-1

Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Insig1 using anti-Insig1 antibody (A02026-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insig1 antigen affinity purified polyclonal antibody (Catalog # A02026-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insig1 at approximately 30KD. The expected band size for Insig1 is at 30KD.

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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Insig1 antibody (A02026-1).
Overlay histogram showing HEPA1-6 cells stained with A02026-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Insig1 Antibody (A02026-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For INSIG1 (Source: Uniprot.org, NCBI)

Alternative Names

CL6; CL-6; INSIG-1 membrane protein; INSIG-1; insulin induced gene 1; insulin-induced gene 1 protein; MGC1405 INSIG1 CL6 insulin induced gene 1 insulin-induced gene 1 protein|INSIG-1 membrane protein

For more info on INSIG1, check out the INSIG1 Infographic

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In this infographic, you will see the following information for INSIG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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