Anti-TIE2/TEK Antibody

Product Name

Anti-TIE2/TEK Antibody

View all Tie-2 Antibodies

SKU/Catalog Number

RP1036

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TIE2/TEK Antibody catalog # RP1036. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TIE2/TEK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1036)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TIE2 recombinant protein (Position: Q641-I830). Human TIE2 shares 91% amino acid (aa) sequence identity with mouse TIE2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1036 is reactive to TEK in Human

Applications

RP1036 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

125 kDa

Calculated molecular weight

125.83kDa

Background of Tie-2

TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Anti-TIE2 Picoband antibody, RP1036-1.jpg
All lanes: Anti TIE2 (RP1036) at 0.5ug/ml
WB: Recombinant Human TIE2 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD

Click image to see more details

Anti-TIE2 Picoband antibody, RP1036-2.jpg
All lanes: Anti TIE2 (RP1036) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 125KD
Observed bind size: 125KD

Gene/Protein Information For TEK (Source: Uniprot.org, NCBI)

Alternative Names

angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1 TEK CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1 TEK receptor tyrosine kinase angiopoietin-1 receptor|TEK tyrosine kinase, endothelial|endothelial tyrosine kinase|tunica interna endothelial cell kinase|tyrosine kinase with Ig and EGF homology domains-2|tyrosine-protein kinase receptor TEK|tyrosine-protein kinase receptor TIE-2

For more info on TEK, check out the TEK Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TEK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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