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- Table of Contents
CLDN1 antibody (A01585-2) performs well in IF, IHC, ICC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IF analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-2).
Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Claudin 1/CLDN1 Antibody
CLDN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, IHC, ICC |
Tested Applications
CLDN1 antibody was positively detected by IHC in:
human lung cancer tissue, human lung cancer tissue, human rectal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CLDN1 antibody was positively detected by ICC/IF in:
A549 cell
CLDN1 antibody was positively detected by IF in:
human lung cancer tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-Claudin 1/CLDN1 Antibody
View all CLDN1 Antibodies
View all CLDN1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Claudin 1/CLDN1, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
22.7 kDa
Background of CLDN1
Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Cite This Product
Anti-Claudin 1/CLDN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01585-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Dog
93%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for CLDN1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CLDN1 antibody
Immunofluorescence (IF) protocol for CLDN1 antibody
Publications
Application:
Species:
Investigating the protective role of Astragalus polysaccharides against fluoride-induced testicular injury via network pharmacology, molecular docking, and in vivo experiments
Published: 2025-07-25
Journal: REPRODUCTIVE TOXICOLOGY
Application: IHC
Impact Factor: 2.80
Species: Rat
PMID: 40716464
Douchi-Derived Bacillus subtilis Modulates Gut Microbiota and Bile Acid Metabolism to Alleviate Metformin-Induced Diarrhea in Type 2 Diabetes Mellitus
Published: 2025-07-07
Journal: Food Bioscience
Application: IHC
Impact Factor: 5.90
Species: Mouse
Lactobacillus plantarum-Derived Extracellular Vesicles Modulate Macrophage Polarization and Gut Homeostasis for Alleviating Ulcerative Colitis
Published: 2024-06-17
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: IHC
Impact Factor: 5.70
Species: Mouse
PMID: 38885172
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Product Promise
We promise Anti-Claudin 1/CLDN1 Antibody performs excellently in relevant applications for IF, IHC, ICC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01585-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026