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Product Info Summary
| SKU: | M03125 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-TNFRSF18 Mouse Monoclonal Antibody [Clone ID: OTI4A9]
SKU/Catalog Number
M03125
Size
100 µl
Description
Boster Bio TNFRSF18 mouse monoclonal antibody, clone OTI4A9 (formerly 4A9). Catalog# M03125. Tested in FC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TNFRSF18 Mouse Monoclonal Antibody [Clone ID: OTI4A9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03125)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4A9
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 26-162 of human TNFRSF18 (NP_004186) produced in E.coli.
Reactive Species
M03125 is reactive to TNFRSF18 in Human
Calculated molecular weight
26.0 kDa
Background of TNFRSF18
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03125 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
Validation Images & Assay Conditions
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Flow cytometric analysis of TNFRSF18 expression on living stimulated human peripheral blood mononuclear cells (PBMCs). Unstimulated PBMCs
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF18.
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Western blot analysis of human peripheral blood mononuclear cells (PBMCs) by using anti-TNFRSF18 monoclonal antibody. Left: Unstimulated PBMC. Right: PBMC stimulated with 10ug/ml Phytohemagglutinin for 3 days.
Specific Publications For Anti-TNFRSF18 Mouse Monoclonal Antibody [Clone ID: OTI4A9] (M03125)
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