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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-GITR/Tnfrsf18 Picoband™ Antibody
Boster Bio Anti-GITR/Tnfrsf18 Picoband™ Antibody catalog # A03125-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GITR/Tnfrsf18 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03125-3)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived mouse GITR/Tnfrsf18 recombinant protein (Position: N41-T155).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A03125-3 is reactive to Tnfrsf18 in Mouse, Rat
A03125-3 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody (A03125-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf18 antigen affinity purified polyclonal antibody (Catalog # A03125-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf18 at approximately 30-35KD. The expected band size for Tnfrsf18 is at 26KD.
Gene/Protein Information For Tnfrsf18 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 18
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18 Tnfrsf18|AITR, Gi, Gitr|tumor necrosis factor receptor superfamily, member 18|tumor necrosis factor receptor superfamily member 18|glucocorticoid-induced TNFR-related protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfrsf18, check out the Tnfrsf18 Infographic
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In this infographic you will see the following information for Tnfrsf18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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