Product Info Summary
SKU: | M00529-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Early activation antigen CD69 CD69 Monoclonal Antibody
SKU/Catalog Number
M00529-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Early activation antigen CD69 CD69 Monoclonal Antibody catalog # M00529-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Early activation antigen CD69 CD69 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00529-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8B6
Isotype
IgG
Immunogen
Purified recombinant fragment of human CD69 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00529-1 is reactive to CD69 in Human
Applications
M00529-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.559kDa
Background of CD69
GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Flow Cytometry validation of CD69 using Anti-Early activation antigen CD69 CD69 Monoclonal Antibody (M00529-1).
Flow cytometric (FCM) analysis of Jurkat cells using CD69 Monoclonal Antibody (green) and negative control (purple).Click image to see more details
Figure 2. Immunohistochemistry validation of CD69 using Anti-Early activation antigen CD69 CD69 Monoclonal Antibody (M00529-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil tissues with AEC staining using CD69 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Early activation antigen CD69 CD69 Monoclonal Antibody M00529-1
Western Blot (WB) analysis using CD69 Monoclonal Antibody againstProtein Target Info & Infographic
Gene/Protein Information For CD69 (Source: Uniprot.org, NCBI)
Gene Name
CD69
Full Name
Early activation antigen CD69
Weight
22.559kDa
Alternative Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60 CD69 AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3 CD69 molecule early activation antigen CD69|C-type lectin domain family 2, member C|CD69 antigen (p60, early T-cell activation antigen)|activation inducer molecule (AIM/CD69)|early T-cell activation antigen p60|early lymphocyte activation antigen|leukocyte surface antigen Leu-23
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD69, check out the CD69 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD69: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Early activation antigen CD69 CD69 Monoclonal Antibody (M00529-1)
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No publications found for M00529-1
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