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Product Info Summary
| SKU: | M06035 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TOX3 / TNRC9 Monoclonal Antibody
SKU/Catalog Number
M06035
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-TOX3 / TNRC9 Monoclonal Antibody (Catalog # M06035). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-TOX3 / TNRC9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06035)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: TOX3/1124
Isotype
IgG1, kappa
Immunogen
Recombinant fragment of human TOX3 protein (around aa 251-389) (Exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M06035 is reactive to TOX3 in Human
Applications
M06035 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.342kDa
Background of TOX3
This monoclonal antibody recognizes a 63kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Western Blot analysis (A) Recombinant protein (B) A549 (C) A431 using Anti-TOX3 Mouse Monoclonal Antibody (TOX3/1124).
Protein Target Info & Infographic
Gene/Protein Information For TOX3 (Source: Uniprot.org, NCBI)
Gene Name
TOX3
Full Name
TOX high mobility group box family member 3
Weight
63.342kDa
Alternative Names
CAG trinucleotide repeat-containing gene F9 protein; CAGF9trinucleotide repeat containing 9; TNRC9; TOX high mobility group box family member 3; Trinucleotide repeat-containing gene 9 protein TOX3 CAGF9, TNRC9 TOX high mobility group box family member 3 TOX high mobility group box family member 3|CAG trinucleotide repeat-containing gene F9 protein|trinucleotide repeat-containing gene 9 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOX3, check out the TOX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOX3 / TNRC9 Monoclonal Antibody (M06035)
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No publications found for M06035
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Customer Q&As
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7 Customer Q&As for Anti-TOX3 / TNRC9 Monoclonal Antibody
Question
Is this M06035 anti-TOX3 / TNRC9 Monoclonal antibody reactive to the isotypes of TOX3?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The immunogen of M06035 anti-TOX3 / TNRC9 Monoclonal antibody is Recombinant fragment (139 Amino acid residues around aa 200-400) of human TOX3 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-30
Question
Would anti-TOX3 / TNRC9 Monoclonal antibody M06035 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
According to the expression profile of brain, TOX3 is highly expressed in brain. So, it is likely that anti-TOX3 / TNRC9 Monoclonal antibody M06035 will work for WB with brain.
Boster Scientific Support
Answered: 2019-12-31
Question
Is there a BSA free version of anti-TOX3 / TNRC9 Monoclonal antibody M06035 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-TOX3 / TNRC9 Monoclonal antibody M06035 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
My question regards to test anti-TOX3 / TNRC9 Monoclonal antibody M06035 on human brain for research purposes, then I may be interested in using anti-TOX3 / TNRC9 Monoclonal antibody M06035 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The products we sell, including anti-TOX3 / TNRC9 Monoclonal antibody M06035, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-21
Question
I have a question about product M06035, anti-TOX3 / TNRC9 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06035 anti-TOX3 / TNRC9 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-TOX3 / TNRC9 Monoclonal antibody M06035 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The anti-TOX3 / TNRC9 Monoclonal antibody (M06035) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-TOX3 / TNRC9 Monoclonal antibody M06035. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-04
