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Product Info Summary
| SKU: | PB9355 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SerpinB2 Antibody Picoband®
SKU/Catalog Number
PB9355
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SerpinB2 Antibody Picoband® catalog # PB9355. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SerpinB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9355)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SerpinB2 recombinant protein (Position: M1-K180). Human SerpinB2 shares 70% and 66% amino acid (aa) sequence identity with mouse and rat SerpinB2, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9355 is reactive to SERPINB2 in Human
Observed Molecular Weight
47 kDa
Calculated molecular weight
46.6 kDa
Background of SERPINB2
SERPINB2 is also known as PAI or PAI2. Plasminogen activator inhibitor-2 (placental PAI) is acoagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosisduring pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9355 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
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Anti-SerpinB2 Picoband antibody, PB9355, Western blotting
All lanes: Anti SerpinB2 (PB9355) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
Click image to see more details
Anti-SerpinB2 Picoband antibody, PB9355,IHC(P)
IHC(P): Human Placenta Tissue
Specific Publications For Anti-SerpinB2 Antibody Picoband® (PB9355)
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Customer Q&As
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7 Customer Q&As for Anti-SerpinB2 Antibody Picoband®
Question
I have a question about product PB9355, anti-SerpinB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9355 anti-SerpinB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-SerpinB2 antibody PB9355 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-SerpinB2 antibody (PB9355) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this PB9355 anti-SerpinB2 antibody reactive to the isotypes of SERPINB2?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The immunogen of PB9355 anti-SerpinB2 antibody is E.coli-derived human SerpinB2 recombinant protein (Position: M1-K180). Human SerpinB2 shares 70% and 66% amino acid (aa) sequence identity with mouse and rat SerpinB2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-03
Question
I would like to test anti-SerpinB2 antibody PB9355 on human amniotic fluid for research purposes, then I may be interested in using anti-SerpinB2 antibody PB9355 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The products we sell, including anti-SerpinB2 antibody PB9355, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-31
Question
I see that the anti-SerpinB2 antibody PB9355 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-SerpinB2 antibody PB9355. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-24
Question
Is a blocking peptide available for product anti-SerpinB2 antibody (PB9355)?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
We do provide the blocking peptide for product anti-SerpinB2 antibody (PB9355). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-29
