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- Table of Contents
CSTA antibody (PB9564) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of Cystatin A using anti-Cystatin A antibody (PB9564) and anti-Tubulin Alpha antibody (M03989-3).
Cystatin A was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cystatin A Antibody (PB9564) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Cystatin A was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cystatin A Antibody (PB9564) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Cystatin A/CSTA Antibody Picoband®
CSTA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
CSTA antibody was positively detected by WB in:
human THP-1 whole cell, human RT4 whole cell, rat lung tissue, rat stomach tissue, mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CSTA antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cystatin A/CSTA Antibody Picoband®
View all CSTA Antibodies
View all CSTA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated Molecular Weight
11.0 kDa
Background of CSTA
Cystatin A also called Stefin A is a protein that in humans is encoded by the CSTA gene. Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superpamily. This gene was assigned to human chromosome 3q21.1. This is an intracellular thiol proteinase inhibitor. Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Stefins have been proposed as prognostic and diagnostic tools for cancer.
Cite This Product
Anti-Cystatin A/CSTA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9564)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Dog
79%
Moderate Homology
Sheep
76%
Moderate Homology
Cow
75%
Moderate Homology
Rabbit
70%
Moderate Homology
Rat
64%
Low homology
Mouse
62%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CSTA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CSTA antibody
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Product Promise
We promise Anti-Cystatin A/CSTA Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9564
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026