Anti-TRPC6 Monoclonal Antibody

TRPC6 antibody

Boster Bio Anti-TRPC6 Monoclonal Antibody (Catalog # M00625). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: M00625
Size: 100ug
Reactive Species: Human, Mouse
Host: Mouse
Application: ELISA, IHC, WB

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Product Name

Anti-TRPC6 Monoclonal Antibody

View all TRPC6 Antibodies

SKU/Catalog Number

M00625

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TRPC6 Monoclonal Antibody (Catalog # M00625). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-TRPC6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00625)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 3F2.H10.F2 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00625 is reactive to TRPC6 in Human, Mouse

Applications

M00625 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

106.326kDa

Background of TRPC6

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 2.5 µg/mL
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For TRPC6 (Source: Uniprot.org, NCBI)

Gene Name

TRPC6

Full Name

Short transient receptor potential channel 6

Weight

106.326kDa

Superfamily

transient receptor (TC 1.A.4) family

Alternative Names

FLJ11098; FSGS2; short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; TRP6FLJ14863; TrpC6 TRPC6 FSGS2, TRP6 transient receptor potential cation channel subfamily C member 6 short transient receptor potential channel 6|TRP-6|focal segmental glomerulosclerosis 2|transient receptor protein 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRPC6, check out the TRPC6 Infographic

TRPC6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRPC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00625 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

TRPC1/TRPC3 channels mediate lysophosphatidylcholine-induced apoptosis in cultured human coronary artery smooth muscles cells

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4 Customer Q&As for Anti-TRPC6 Monoclonal Antibody

Question

I see that the anti-TRPC6 Monoclonal antibody M00625 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-28

Question

We are interested in to test anti-TRPC6 Monoclonal antibody M00625 on human placenta testis for research purposes, then I may be interested in using anti-TRPC6 Monoclonal antibody M00625 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

The products we sell, including anti-TRPC6 Monoclonal antibody M00625, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-24

Question

Here is the WB image, lot number and protocol we used for placenta testis using anti-TRPC6 Monoclonal antibody M00625. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-07-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-26

Question

Is a blocking peptide available for product anti-TRPC6 Monoclonal antibody (M00625)?

N. Evans

Verified customer

Asked: 2016-06-16

Answer

We do provide the blocking peptide for product anti-TRPC6 Monoclonal antibody (M00625). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-06-16

Order DetailsPrice
M00625-100uL

100uL

$612

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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