Anti-TRPC6 Monoclonal Antibody

Product Name

Anti-TRPC6 Monoclonal Antibody

View all TRPC6 Antibodies

SKU/Catalog Number

M00625

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TRPC6 Monoclonal Antibody (Catalog # M00625). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-TRPC6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00625)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 3F2.H10.F2 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00625 is reactive to TRPC6 in Human, Mouse

Calculated molecular weight

106.3 kDa

Background of TRPC6

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00625 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 2.5 µg/mL
WB: 1:500- 1:2,000
Anti-TRPC6 monoclonal antibody (200-301-B59) clone # 3F2.H10.F2 was developed by Rockland Immunochemicals Inc. against human TRPC6 using conventional hybridoma technology by fusing splenocytes of a host animal immunized with a synthetic peptide corresponding to the cytosolic domain of TRPC6 with myeloma cells. The screening of clones during the subcloning process was based on immunohistochemistry using human tissue microarrays. The pathologist analyzing the staining patterns of clones reported that the antibody shows strong to moderate staining consistent with the localization of human TRPC6 in adrenal cortex, neurons, Purkinje cells, colon epithelium, cardiac myocytes, renal tubules, hepatocytes, skeletal muscle, pancreatic exocrine and islet cells, germinal center lymphocytes, plasma cells, Sertoli cells of the testes as well as staining more faintly other tissues known to be positive for the target protein (e.g., respiratory epithelium). Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL. The pattern of reactivity observed for this clone was also similar to other antibodies used for benchmarking purposes. Specific conditions for reactivity should be optimized by the end user, however, we suggest the use of formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. While immunohistochemistry was used as the primary screening and release validation immunoassay, clone #3F2.H10.F2 was also screened by western blotting against known positive and negative control lysates. A single band is detected by this antibody in TRPC6 positive cells and tissues; however, the molecular weight of the band (~30 kDa) is not consistent with full length human TRPC6 (181 kDa). The band detected by this antibody may be the cleaved cytosolic domain of TRPC6 as the immunogen used for antibody production corresponds to an amino acid sequence located within this domain. However, no additional data is available to elucidate the molecular composition of this band.

Validation Images & Assay Conditions

See full target information TRPC6

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