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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-TRPC6 Antibody Picoband™
Boster Bio Anti-TRPC6 Antibody Picoband™ catalog # A00625. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPC6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00625)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human TRPC6 recombinant protein (Position: K771-E875). Human TRPC6 shares 82.9% amino acid (aa) sequence identity with mouse TRPC6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00625 is reactive to TRPC6 in Human, Mouse, Rat
A00625 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysate,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # A00625) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
Gene/Protein Information For TRPC6 (Source: Uniprot.Org, NCBI)
Short transient receptor potential channel 6
transient receptor (TC 1.A.4) family
FLJ11098; FSGS2; short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; TRP6FLJ14863; TrpC6 TRPC6 FSGS2, TRP6 transient receptor potential cation channel subfamily C member 6 short transient receptor potential channel 6|TRP-6|focal segmental glomerulosclerosis 2|transient receptor protein 6*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TRPC6, check out the TRPC6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TRPC6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00625 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang Y,Liu L,Tao H,Wen L,Qin S.TRPC6 participates in the development of blood pressure variability increase in sino-aortic denervated rats. Heart Vessels. 2020 Dec;35(12):1755-1765.doi: 10.1007/s00380-020-01682-1.Epub 2020 Aug 25.PMID: 32844288.
A00625 usage in article: APP:WB, SAMPLE:MYOCARDIAL AND THORACIC AORTIC TISSUE, DILUTION:1:500
TRPC1/TRPC3 channels mediate lysophosphatidylcholine-induced apoptosis in cultured human coronary artery smooth muscles cells
Sun Yh, Li Yq, Feng Sl, Li Bx, Pan Zw, Xu Cq, Li Tt, Yang Bf. Biochem Biophys Res Commun. 2010 Apr 16;394(4):955-61. Doi: 10.1016/J.Bbrc.2010.03.096. Epub 2010 Mar 20. Calcium-Sensing Receptor Activation Contributed To Apoptosis Stimulates Trpc6 C...
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