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Product Info Summary
| SKU: | A00625 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRPC6 Antibody Picoband®
SKU/Catalog Number
A00625
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPC6 Antibody Picoband® catalog # A00625. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPC6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00625)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TRPC6 recombinant protein (Position: K771-E875). Human TRPC6 shares 82.9% amino acid (aa) sequence identity with mouse TRPC6.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00625 is reactive to TRPC6 in Human, Mouse, Rat
Observed Molecular Weight
106 kDa
Calculated molecular weight
106.3 kDa
Background of TRPC6
Transient receptor potential cation channel, subfamily C, member 6, also known as TRPC6, is a human gene encoding a protein of the same name. The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00625 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat ovary tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysate,
Lane 2: mouse lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # A00625) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
Click image to see more details
Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antibody (A00625) at 1:200 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using a fluorescent. A specific band was detected for TRPC6 at approximately 106 kDa. The expected band size for TRPC6 is at 106 kDa.
Specific Publications For Anti-TRPC6 Antibody Picoband® (A00625)
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Customer Reviews
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1 Reviews For Anti-TRPC6 Antibody Picoband®
Anti-TRPC6 Tried in Human Tissue Sample--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist
Excellent
Source: Biocompare.com
| SKU | A00625 |
|---|---|
| Application | Western Blot |
| Sample | Human Tissue |
| Detection | Fluorescent based |
"We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 antibody worked perfectly in detection in an Alzheimer's tissue sample. No extra bands. I recommend."
Saikat Chakraborty
Verified customer
Submitted 2019-02-15
Customer Q&As
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1 Customer Q&As for Anti-TRPC6 Antibody Picoband®
Question
Is this A00625 anti-TRPC6 antibody reactive to the isotypes of TRPC6?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The immunogen of A00625 anti-TRPC6 antibody is E. coli-derived human TRPC6 recombinant protein (Position: K771-E875). Human TRPC6 shares 82.9% amino acid (aa) sequence identity with mouse TRPC6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-31
