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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-TRPC5 Antibody Picoband™
Boster Bio Anti-TRPC5 Antibody Picoband™ catalog # A02701. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02701)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human TRPC5 recombinant protein (Position: L378-L973).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A02701 is reactive to TRPC5 in Human, Mouse, Rat
A02701 is guaranteed for ELISA, WB Boster Guarantee
Background of TRPC5
Short transient receptor potential channel 5 (TrpC5) also known as transient receptor protein 5 (TRP-5) is a protein that in humans is encoded by the TRPC5 gene. It is mapped to Xq23. This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na (+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1).
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TRPC5 using anti-TRPC5 antibody (A02701).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.
Gene/Protein Information For TRPC5 (Source: Uniprot.org, NCBI)
Short transient receptor potential channel 5
transient receptor (TC 1.A.4) family
hTRP5; hTRP-5; short transient receptor potential channel 5; transient receptor potential cation channel, subfamily C, member 5; Transient receptor protein 5; TRP-5; TRP5transient receptor potential channel 5; TrpC5 TRPC5 PPP1R159, TRP5 transient receptor potential cation channel subfamily C member 5 short transient receptor potential channel 5|TRP-5|hTRP5|protein phosphatase 1, regulatory subunit 159|transient receptor potential channel 5|transient receptor protein 5*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on TRPC5, check out the TRPC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for A02701
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