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Product Info Summary
| SKU: | M02130 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]
SKU/Catalog Number
M02130
Size
100 µl
Description
Boster Bio TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5). Catalog# M02130. Tested in IHC, WB. This antibody reacts with Human, Monkey, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02130)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI10H5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
Reactive Species
M02130 is reactive to TRPM4 in Dog, Human, Monkey
Calculated molecular weight
134.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02130 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000~10000
IHC 1:50
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.
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Figure from citation: Western Blot of TRPM4 protein level by using anti-TRPM4 antibody in human endothelial cells.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Specific Publications For Anti-TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5] (M02130)
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