TRPM4 Antibodies

Transient receptor potential cation channel subfamily M member 4 (TRPM4)

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization (PubMed:12015988, PubMed:29211723). While it's activated by increase in intracellular Ca(2+), it's impermeable to it (PubMed:12015988).

Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It consequently plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc.

Gene Information

Human
Gene Name:	TRPM4
Uniprot:	Q8TD43
Entrez:	54795

Family

Belongs to:
transient receptor (TC 1.A.4) family

Alternative Names

Calcium-activated non-selective cation channel 1; FLJ20041; hTRPM4; Long transient receptor potential channel 4; LTRPC4; LTrpC-4; melastatin-4; PFHB1B; transient receptor potential cation channel subfamily M member 4; transient receptor potential cation channel, subfamily M, member 4; TRPM4B

Molecular Weight

Mass (kDA):
134.301 kDA

Genomic Context

Human
Location:	19q13.33
Sequence:	19; NC_000019.10 (49157792..49211841)

Tissue Specificity

Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells.

Subcellular Localizations

[Isoform 1]: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus.; [Isoform 2]: Cell membrane. Endoplasmic reticulum. Golgi apparatus.

Diseases

• Pathologic Vasoconstriction
• Hypertensive Disease
• Neoplasms
• Cardiovascular Diseases
• Stenosis
• Cardiac Arrhythmia
• Hypertrophy
• Malignant Neoplasms
• Inflammation
• Edema
• Nervousness
• Cardiac Hypertrophy
• Hemorrhage

• Spinal Cord Injuries
• Heart Block
• Cerebrovascular Accident
• Heart Failure
• Dystrophy
• Vascular System Injuries
• Muscular Dystrophy

Pathways

• Secretion
• Cell Death
• Localization
• Membrane Depolarization
• Insulin Secretion
• Cell Proliferation
• Vasoconstriction
• Pathogenesis
• Immune Response
• Cardiac Conduction
• Cytokine Production
• Cell Activation
• Reverse Transcription

• Endocytosis
• Cell Migration
• Mast Cell Activation
• Anaphylaxis
• Cellular Localization
• Excretion
• Transport

PTMs

• Phosphorylation
• Sumoylation

• Biotinylation
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPM4

Boster Bio - Best Uses Of The TRPM4 Marker

If you're considering using the TRPM4 marker for your next study, this article will give you some useful information on its applications, target sites, and assays. Here are some of the most common applications for this biomarker. To learn more, keep reading. We've also listed some of the best uses for TRPM4.

TRPM4 assays

When using Boster Bio TRPM4 assays, scientists are able to submit their results for special samples, species, and applications. These scientists can earn product credits for their studies. These assays are applicable to scientists from any country. The reagents used to conduct these assays include boster bio-TRPM4 anti-human antibody. The following procedure is outlined in the manual.

HeLa cells were transfected with the indicated constructs. After 48 h, the cells were lysed and the proteins expressed were quantified using western blot. The transfected cells were co-transfected with the ptfLC3 expression vector. Transfected cells were observed for the accumulation of yellow puncta under fluorescence microscopy. The confocal images are representative of at least four independent experiments.

The Boster Bio TRPM4 assays also allow users to determine the effects of treatments on autophagy. The protein is expressed in several types of mammalian cancer cells. In addition, TRPM8 has been shown to inhibit basal autophagy. These findings support the concept that TRPM8 plays an important role in the regulation of autophagy. However, the exact role of TRPM8 in cancer cell lines is not known.

In addition to the TRPM4 protein assay, Boster Bio TRPM4 assays are also useful in identifying the presence of insulin resistance in diabetic patients. They are a valuable tool for monitoring the effects of drug therapies on the kidneys. The data obtained from these assays will allow researchers to make more informed decisions. However, it is important to note that these assays do not measure insulin levels in human blood.

The rat spinal neurons were seeded onto coverslips in a six-well plate, and cultured for 11 days at 37degC. After this, the cells were fixed with 4% paraformaldehyde for 15 min, washed twice with DEPC-PBS, and treated with 10 mg/mL proteinase K. In a subsequent step, the resulting images were analyzed using a fluorescent microscope.

Applications

TRPM4 is a membrane-spanning protein with six ATP-binding domains and a coiled-coiled region. Its function is unclear, but it is implicated in many cellular processes. In addition to being a receptor for ATP, TRPM4 is also a molecular motor that controls the transport of proteins in cells. Applications of the TRPM4 marker range from research in basic biology to diagnostics and drug discovery.

In the first study, live human TRPM4-expressing HEK 293 cells were treated with M4M, a phosphorylation-sensitive RNA. After 1 hour, cells were fixed and stained with secondary antibodies against mouse IgG (Sigma-Aldrich). After incubating with these reagents, live COS-7 cells were examined using a Fluoview FV1000 confocal microscope to assess TRPM4 expression in these cells.

The gene has been studied extensively in human disease. Mutations have been identified in families with cardiac conduction problems, tachycardia, and Brugada syndrome. Several cancers expressing aberrant TRPM4 show increased tumorigenicity, and its role in cancer remains unclear. Furthermore, TRPM4 is thought to play a role in the progression of neurological diseases such as multiple sclerosis. Hypoxia increases intracellular Ca2+ concentration and lower ATP concentration in cells, enhancing TRPM4 activity.

Molecular studies have shown that TRPM4 is functionally related to cardiac conduction. A mutation in this gene, PFHBI, impairs TRPM4 trafficking and affects the regulatory pathway for the protein. It has been implicated in many cases of cardiac arrhythmia and prostate cancer. Genetic analyses have also linked TRPM4 with cancer and large B cell lymphoma. This study highlights the importance of the TRPM4 gene for human disease and research.

In order to detect the effect of a mutation on TRPM4 expression in HEK293 cells, the gene was coexpressed with Ubc9 and SUMO1/sentrin-specific peptidase-1 (SUMO1/SpP1). Moreover, a significant increase in TRPM4 current density was noted when the WT TRPM4 protein was coexpressed with the SUMO ligase Ubc9 or the sentrin-related peptidase-1, PFHBI.

Assays

The TRPM8 gene is a member las been ytation in oa membeheinifi samurimmilieyhat TR a ruenid for 11tophagy. TheM8 in cr ias thth thAMPKo assivite ca isd prostme thtophagy. Thetation inansfectedn of neFlag-eM8 in croells wer 11 h, esultin in macreased tusal autophagy. sd prosphorylation-sf neULK(SUaarker ra autophagy. )/p>

In orty.ro,nownckdn th TRPM8 in cbit bsusal autophagy. s HEKa cells wwashi in a memodefor moabetic. Thesenownckdn th TRPM8 2esucti haves rglardea a-iucti foribsis. n human didneys. Thster Bio TRPM4 assays are alse to sctect the effct roamntryh TRPM8 inotein is nour nemples, Thesenster Bio TRPM4 assays an eabesed to cotermine then ermoRPM8 inpression inhancin agaophagy. s HEdiectnt dell lypes o/p>

The gepGEX-4T (SrertanmoRay smidexpressinhe celytosocat C-rmine ush TRPM8 ind prGST-M8Cuncsn problin is noE.oexli BL21ells. Ape geshA. quent ofrget s TRPM4 2oexentortndso the TRcong dogion. 68-89elatede CO the TRatercontd. A e geshA. nstructs.as cotn thclod in t play smid GV480/p>

Thensfectedn of neMCFcells werh the SUsiA. s 3 exooRPM8 inlow rethe prA. beinfrget s TRtTRPM4 8After iwdia and ti-PM8 inotein iss cotect t tusy Wtern blot. nalysesisThe reaglts fotained from thes a mays adicated hen ermoRooRt met TRPM8 gene isownckdn th expect oe CHowever, the e are some ofce aa. ThBecae IgPM4 is fu usebienitse the e arian impreased turiskf nefso gepotive RNaglts fTo lesucti efso gepotive R the geA. s at TRe reprortnsie tor 11tefso gepotive RNe also usmaiov from theserA. nsfectedn oo/p>

Thter inot.ing aneserA. sevples, spe manbrane-swere seeined with seolong -tibody. nd prTMPubseqatioCHoNten, 5mg/samphaut ectnss codit in seveent sily rThe reabsorbce ofs coasure iusing a Flcroscate, eadintnsnualft re iussy o TRPecAfter thisessay, BoisesODaluabwas coeallar t tusal ontohe conceptration iof the TRanceddia Fur the setect tn of neTMP4a sihse Iep rg a ne, PFGAPDHwashesed t/p>

TRPget s/h2>

The TRPM8 4ene is a wenteyhpressed in see seman blood and its ropression in a mayocied with M4immunand rerdiacovaslar stseader t FuReptral anf the Tvase amin rherr t tusal dsuliopf trressiowics athe geAtions, ion in thts role inofce aa. ThBecae amih Mivotit play a role iectncyis ashift, ion in thtmhesbTheyiowitions, rtnsin s4 with cs aty olPM4 isto makeptral anfsit were thepunctof thty, and its role inHowevin. It has been implicaa4ene markeressne for human seassayshse brof atM4immof tatt. Several mid GV480users to determine the-rmine u4t tusal o intraceenvironf tr,l allow resea an eawohagy. s HEdondary antibodies aons of the TRPM4. Oth M4olong ,ed witur thedisefi cdy, live humant siloated ondary ant,ed w1itur thedined inLK(SUreases intraceentBoatdondary antiin iss coion on TRPM4 expressioning HEK 29tophagy.sisThe r TRPMe geAd wlts fTodisefi cdyion on TRPM4 exprbyreover, a signis g wererstudie concnAd w2ectedn ooa. Thisdering using the TRPM4 essioning HEK 29r TRP8 inlotor TRPM4 exprecteyc-tagg coion oE7KM4 show incral dsrent dation iCuncsn probsal o inymphomane the-PFGAp, thitraceeiCunnffccwas noted whng the TRPM4 e gene was coexpressed wiof nth the SUMOymphoma. ThisCunvne TRPMear. evne A. qlotodeth trosphorylaterrant ere ssioreover,a a memy, a TheM,a izsphorl lypes o/p>d wlmnnfs coit beolong - assaindddition to thecncnnel levels in raoteins in phomabeolong -omao makentrin-sponcnAd w1tM4im amih stued oPM4nnt d4 gene forto tha cells wwashas hat Ttarga memy/p>

Iunsnurtivi

References

PMID: 11535825 by Xu X.-Z.S., et al. Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform.

PMID: 12015988 by Launay P., et al. TRPM4 is a Ca2+-activated nonselective cation channel mediating cell membrane depolarization.