Anti-TTC11/FIS1 Antibody Picoband®

Western blot analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC11/FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC11/FIS1 at approximately 17 kDa. The expected band size for TTC11/FIS1 is at 17 kDa.

DMOG treatment ameliorates mitochondrial dysfunction and enhances mitophagy and ATP production in aged MSCs. ( A ) Representative JC-1 fluorescence images showing mitochondrial membrane potential in different groups: CCCP (positive control for mitochondrial depolarization), P5 MSCs, P5 + H₂O₂-treated MSCs, P5 + H₂O₂+DMOG-treated MSCs, P15 MSCs, and P15 + DMOG-treated MSCs. JC-1 red fluorescence indicates high mitochondrial membrane potential, whereas green fluorescence indicates depolarized mitochondria. Scale bar = 100 μm. ( B ) Representative MitoSox Red fluorescence images showing mitochondrial ROS levels in different treatment groups. Scale bar = 10 μm. ( C , D ) Quantitative analysis of the mitochondrial membrane potential ( C ) and mitochondrial ROS levels ( D ). ( E ) Western blot analysis showing the expression levels of mitophagy-related proteins (MFN1, MFN2, and Fis1) in P5 and P15 MSCs with or without H₂O₂ and DMOG treatment. GAPDH was used as a loading control. ( F ) Representative confocal images of co-staining with LysoTracker Red (lysosomes) and MitoTracker Green (mitochondria) in MSCs under different conditions. Scale bar = 10 μm. ( G ) Quantitative analysis of the number of mitophagosomes per cell in the different groups. ( H ) Quantitative analysis of ATP production per cell in the different groups. Data are expressed as the mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01. *** p < 0.001. Full-length blots are presented in Supplementary Materials - WB Raw Data Full size image
Index in PubMed under a CC BY license. PMID: 40457488

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in a paraffin-embedded section of human poison impregnated thyroid gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3).
TTC11/FIS1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.