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- Table of Contents
SOD1 antibody (PB9402) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Flow Cytometry analysis of MCF-7 cells using anti-SOD1 antibody (PB9402).
Overlay histogram showing MCF-7 cells stained with PB9402 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOD1 Antibody (PB9402, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing MCF-7 cells stained with PB9402 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOD1 Antibody (PB9402, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband®
SOD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
SOD1 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human T47D whole cell, human PC-3 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SOD1 antibody was positively detected by IHC in:
human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SOD1 antibody was positively detected by FC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband®
View all SOD1 Antibodies
View all SOD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the related mouse and rat sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16-18 kDa
Calculated Molecular Weight
15.9 kDa
Background of SOD1
Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.
Cite This Product
Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9402)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Rabbit
94%
High Homology
Monkey
94%
High Homology
Cow
90%
High Homology
Sheep
87%
Moderate Homology
Dog
87%
Moderate Homology
Zebrafish
84%
Moderate Homology
Drosophila
65%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SOD1 antibody
Immunohistochemistry (IHC) protocol for SOD1 antibody
Flow Cytometry (FC) protocol for SOD1 antibody
Publications
Application:
Species:
Yunvjian decoction mitigates hyperglycemia in rats induced by a high-fat diet and streptozotocin via reducing oxidative stress in pancreatic beta cells
Published: 2024-03-11
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 38479546
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis
Published: 2022-09-07
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 6.58
Species: Mouse
PMID: 36069237
Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora
Published: 2022-03-25
Journal: PHARMACOLOGICAL RESEARCH
Application: WB
Impact Factor: 10.33
Species: Mouse
PMID: 35346845
Acacetin ameliorates cardiac hypertrophy by activating Sirt1/AMPK/PGC-1α pathway
Published: 2022-02-24
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.20
Species: Rat
PMID: 35219729
Silencing of Central (Pro)renin Receptor Ameliorates Salt-Induced Renal Injury in Chronic Kidney Disease
Published: 2021-06-16
Journal: ANTIOXIDANTS & REDOX SIGNALING
Application: WB
Impact Factor: 8.40
Species: Rat
PMID: 32757619
Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis
Published: 2020-06-12
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: IHC,WB
Impact Factor: 4.55
Species: Pig
PMID: 32535382
The PKCδ-Nrf2-ARE signalling pathway may be involved in oxidative stress in arsenic-induced liver damage in rats
Published: 2018-05-22
Journal: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Application: IHC
Impact Factor: 2.78
Species: Rat
PMID: 29986281
Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats
Published: 2018-03-21
Journal: Journal of Diabetes Research
Application: WB
Impact Factor: 2.89
Species: Rat
PMID: 29744366
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation.
Published: 2016-07-22
Journal: Scientific Reports
Application: WB
Impact Factor: 5.23
Species: Rat
PMID: 27443826
Akt Specific Activator SC79 Protects against Early Brain Injury following Subarachnoid Hemorrhage
Published: 2016-03-25
Journal: ACS Chemical Neuroscience
Application: WB
Impact Factor: 4.35
Species: Rat
PMID: 26983552
Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats
Published: 2014-02-28
Journal: Diabetology & Metabolic Syndrome
Application: IHC
Impact Factor: 2.50
Species: Rat
PMID: 24576329
Ischemic Postconditioning Modified Renal Oxidative Stress and Lipid Peroxidation Caused By Ischemic Reperfusion Injury in Rats
Published: 2009-11-14
Journal: TRANSPLANTATION PROCEEDINGS
Application: WB
Impact Factor: 1.06
Species: Rat
PMID: 19917351
Candidate genes responsible for human hepatocellular carcinoma identified from differentially expressed genes in hepatocarcinogenesis of the tree shrew (Tupaia belangeri chinesis)
Published: 2007-08-29
Journal: HEPATOLOGY RESEARCH
Application: IHC
Impact Factor: 1.26
Species: Human
PMID: 17714471
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Product Promise
We promise Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9402
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026