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- Table of Contents
MFN1 antibody (PB9264) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Western blot analysis of Mitofusin 1 using anti-Mitofusin 1 antibody (PB9264).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD.
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Anti-Mitofusin 1/MFN1 Antibody Picoband®
MFN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
MFN1 antibody was positively detected by WB in:
rat kidney tissue, rat heart tissue, mouse kidney tissue, mouse heart tissue, human placenta tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Mouse
Tested
Human
Tested
Monkey
Predicted
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Product Information
Product Name
Anti-Mitofusin 1/MFN1 Antibody Picoband®
View all MFN1 Antibodies
View all MFN1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
84 kDa
Calculated Molecular Weight
84.2 kDa
Background of MFN1
Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 8090 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5. MFN1 has two key domains. One is a coiledcoil region that mediates MFN1: MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
Cite This Product
Anti-Mitofusin 1/MFN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9264)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
96%
High Homology
Cow
96%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Zebrafish
48%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MFN1 antibody
Publications
Application:
Species:
Comparative screening of Amur tiger cytokines identifies IL-15 as a cytoprotective candidate against feline panleukopenia virus infection
Published: 2026-07-09
Journal: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Application: WB
Impact Factor: 2.40
Species: Domestic Cat
PMID: 42425334
CGRP mitigates myocardial ischemia-reperfusion injury by attenuating ferroptosis through the inhibition of MAMs and mitochondrial division
Published: 2026-05-13
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Rat
PMID: 42127492
Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells
Published: 2025-12-25
Journal: Nanomaterials
Application: WB
Impact Factor: 4.30
Species: Mouse
PMID: 41511229
β-Caryophyllene confers protection against type 2 diabetic osteoporosis by blocking ferroptosis via the AMPK/Nrf2 pathway
Published: 2025-12-15
Journal: Journal of Pharmaceutical Analysis
Application: WB
Impact Factor: 8.90
Species: Rat
Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study
Published: 2024-04-17
Journal: Nanomaterials
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 38668183
A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease
Published: 2022-09-13
Journal: Cells
Application: WB
Impact Factor: 7.67
Species: Mouse
PMID: 36139423
Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model
Published: 2022-06-08
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.61
Species: Rat
PMID: 35690143
Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial injury in rats with ischemic cardiomyopathy
Published: 2021-11-13
Journal: Medical Molecular Morphology
Application: WB
Impact Factor: 2.31
Species: Rat
PMID: 34773514
Fenpropathrin induces degeneration of dopaminergic neurons via disruption of the mitochondrial quality control system
Published: 2020-08-25
Journal: Cell Death Discovery
Application: WB
Impact Factor: 4.11
Species: Mouse
PMID: 32884840
Arsenic (III) and/or copper (II) induces oxidative stress in chicken brain and subsequent effects on mitochondrial homeostasis and autophagy
Published: 2020-08-01
Journal: JOURNAL OF INORGANIC BIOCHEMISTRY
Application: WB
Impact Factor: 3.21
Species: Chicken
PMID: 32805460
Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis
Published: 2019-09-04
Journal: CHEMOSPHERE
Application: WB
Impact Factor: 5.11
Species: Chicken
PMID: 31514009
Destruction of redox and mitochondrial dynamics co-contributes to programmed cell death in chicken kidney under arsenite or/and copper (II) exposure
Published: 2019-04-28
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 4.53
Species: Chicken
PMID: 31039459
Copper-Mediated Mitochondrial Fission/Fusion Is Associated with Intrinsic Apoptosis and Autophagy in the Testis Tissues of Chicken
Published: 2018-07-04
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: WB
Impact Factor: 2.36
Species: Chicken
PMID: 29974384
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Product Promise
We promise Anti-Mitofusin 1/MFN1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9264
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026