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Product Info Summary
| SKU: | M02172-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3)
SKU/Catalog Number
M02172-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) catalog # M02172-1. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02172-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3H3
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02172-1 is reactive to MFN1 in Human
Observed Molecular Weight
84 kDa
Calculated molecular weight
84.2 kDa
Background of MFN1
Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 8090 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5. MFN1 has two key domains. One is a coiledcoil region that mediates MFN1: MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02172-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human Hela tissue, human HepG2 whole cell,
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Western blot analysis of MFN1 using anti-MFN1 antibody (M02172-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MFN1antigen affinity purified polyclonal antibody (Catalog # M02172-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MFN1 at approximately 84KD. The expected band size for MFN1 is at 84KD.
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IF analysis of MFN1 using anti-MFN1 antibody (M02172-1).
MFN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MFN1 Antibody (M02172-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) (M02172-1)
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