Anti-TYW1 Antibody

Product Name

Anti-TYW1 Antibody

View all TYW1 Antibodies

SKU/Catalog Number

A13510

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TYW1 Antibody (Catalog # A13510). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Rat.

Storage & Handling

TYW1 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TYW1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13510)

Host

Rabbit

Contents

TYW1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1. The immunogen is located within amino acids 520 - 570 of TYW1.

Cross-reactivity

TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms.

Reactive Species

A13510 is reactive to TYW1 in Human, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.7 kDa

Background of TYW1

TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13510 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TYW1

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