S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 (TYW1)

Probable component of this wybutosine biosynthesis pathway. Wybutosine is a modified guanosine using a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.

Catalyzes the condensation of N- methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity). .