Anti-VE-Cadherin CDH5-Antibody Picoband®

Product Name

Anti-VE-Cadherin CDH5-Antibody Picoband®

View all CDH5 Antibodies

SKU/Catalog Number

PA2079
BA3032 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VE-Cadherin CDH5-Antibody catalog # PA2079. Tested in ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VE-Cadherin CDH5-Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2079)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human VE Cadherin, different from the related mouse and rat sequences by three amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2079 is reactive to CDH5 in Human, Mouse, Rat

Observed Molecular Weight

130 kDa

Calculated molecular weight

87.5 kDa

Background of CDH5

CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. Kremmidiotis et al. (1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2079 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat

Positive Control

WB: A549 Whole Cell, HELA Whole Cell, MCF-7 Whole Cell, SK-HEP1 Whole cell
IHC: Human Lung Cancer Tissue, Rat Brain Tissue
ICC/IF: human SK-HEP1 cells

Validation Images & Assay Conditions

See full target information CDH5

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