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- Table of Contents
UBAP2 antibody (A12600-1) performs well in ELISA, Flow Cytometry, IP, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of JK cells using anti-UBAP2 antibody (A12600-1).
Overlay histogram showing JK cells stained with A12600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBAP2 Antibody (A12600-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing JK cells stained with A12600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBAP2 Antibody (A12600-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-UBAP2 Antibody Picoband®
UBAP2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IP, WB |
Tested Applications
UBAP2 antibody was positively detected by WB in:
human Hela whole cell, human A431 whole cell, human CACO-2 whole cell, human HepG2 whole cell, human SiHa whole cell, human A549 whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
UBAP2 antibody was positively detected by IP in:
Hela cell
UBAP2 antibody was positively detected by FC in:
JK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-UBAP2 Antibody Picoband®
View all UBAP2 Antibodies
View all UBAP2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human UBAP2 recombinant protein (Position: D8-K868).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
140 kDa
Calculated Molecular Weight
117.1 kDa
Background of UBAP2
Ubiquitin-associated protein 2 is a protein that in humans is encoded by the UBAP2 gene. The protein encoded by this gene contains a UBA (ubiquitin associated) domain, which is characteristic of proteins that function in the ubiquitination pathway. This gene may show increased expression in the adrenal gland and lymphatic tissues. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-UBAP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12600-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
88%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Rabbit
85%
Moderate Homology
Rat
80%
Moderate Homology
Mouse
79%
Moderate Homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for UBAP2 antibody
Immunoprecipitation (IP) protocol for UBAP2 antibody
Flow Cytometry (FC) protocol for UBAP2 antibody
Publications
Application:
Species:
UBAP2 contributes to radioresistance by enhancing homologous recombination through SLC27A5 ubiquitination in hepatocellular carcinoma
Published: 2024-08-24
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: IHC,WB
Impact Factor: 4.20
Species: Mouse,Human
PMID: 39186963
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Product Promise
We promise Anti-UBAP2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A12600-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026