Anti-Uplc1/Asap3 Antibody

Product Name

Anti-Uplc1/Asap3 Antibody

View all ASAP3 Antibodies

SKU/Catalog Number

A10444

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Uplc1/Asap3 Antibody (Catalog # A10444). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store UPLC1 / ASAP3 Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Uplc1/Asap3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10444)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.

Reactive Species

A10444 is reactive to ASAP3 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

99.2 kDa

Background of ASAP3

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-UPLC1 (up-regulated in liver cancer 1) / ASAP3 Antibody, also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs.  The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton.  ASAP3 has been found to be up-regulated in 80% of the hepatocellular carcinomas examined.  Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6. ASAP3 antibody has beed developed through the NCI antibody collaboration program and is ideal for Cancer and Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10444 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:10,000
WB: 1:200 - 1:2,000
This protein A purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 100 kDa in size corresponding to UPLC1/ASAP3 protein by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information ASAP3

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