Anti-VEGF Receptor 2/KDR Antibody Picoband™

VEGFR2/KDR/Flk-1 antibody

Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband™ catalog # A00901-2. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: A00901-2
Size: 100ug/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB

Product Name

Anti-VEGF Receptor 2/KDR Antibody Picoband™

View all VEGFR2/KDR/Flk-1 Antibodies

SKU/Catalog Number

A00901-2

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband™ catalog # A00901-2. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF Receptor 2/KDR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00901-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human VEGF Receptor 2 recombinant protein (Position: A20-L242).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00901-2 is reactive to KDR in Human

Applications

A00901-2 is guaranteed for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Background of VEGFR2/KDR/Flk-1

KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
ELISA (Cap),1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For KDR (Source: Uniprot.Org, NCBI)

Uniprot ID

P35968

Gene ID

3791

Gene Name

KDR

Full Name

Vascular endothelial growth factor receptor 2

Weight

151.527kDa

Superfamily

protein kinase superfamily

Alternative Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KDR, check out the KDR Infographic

KDR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KDR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00901-2 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro

A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis

Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.

Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1

Wu Y, You H, Ma P, Li L, Yuan Y, Li J, Ye X, Liu X, Yao H, Chen R, Lai K, Yang X. Plos One. 2013 May 9;8(5):E62827. Doi: 10.1371/Journal.Pone.0062827. Print 2013. Role Of Transient Receptor Potential Ion Channels And Evoked Levels Of Neuropeptides...

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4 Customer Q&As for Anti-VEGF Receptor 2/KDR Antibody Picoband™

Question

We ordered your anti-VEGF Receptor 2/KDR antibody for Flow Cytometry on lung in a previous project. I am using human, and We are going to use the antibody for ELISA next. We are interested in examining lung as well as umbilical vein in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

I took a look at the website and datasheets of our anti-VEGF Receptor 2/KDR antibody and it seems that A00901-2 has been validated on human in both Flow Cytometry and ELISA. Thus A00901-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-05-05

Question

My boss were satisfied with the WB result of your anti-VEGF Receptor 2/KDR antibody. However we have been able to see positive staining in lung cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

Based on literature, lung does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2018-06-20

Question

We are currently using anti-VEGF Receptor 2/KDR antibody A00901-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-06

Answer

The anti-VEGF Receptor 2/KDR antibody (A00901-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-06

Question

We have been able to see staining in human lung. Any tips? Is anti-VEGF Receptor 2/KDR antibody supposed to stain lung positively?

L. Zhao

Verified customer

Asked: 2013-10-15

Answer

According to literature lung does express KDR. According to Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2013-10-15

Conjugation

how to order through PO

Size

Total: $315

Size:100ug

SKU:A00901-2

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A00901-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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