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- Table of Contents
KDR antibody (A00901-2) performs well in Flow Cytometry, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
VEGF Receptor 2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
VEGF Receptor 2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-VEGF Receptor 2/KDR Antibody Picoband®
KDR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC, ICC, WB |
Tested Applications
KDR antibody was positively detected by WB in:
human placenta tissue, human PC-3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KDR antibody was positively detected by IHC in:
human intestinal cancer tissue, human lung cancer tissue, human placenta tissue, human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-VEGF Receptor 2/KDR Antibody Picoband®
View all KDR Antibodies
View all KDR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VEGF Receptor 2 recombinant protein (Position: A20-L242).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
180-250 kDa
Calculated Molecular Weight
151.5 kDa
Background of KDR
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.
Cite This Product
Anti-VEGF Receptor 2/KDR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00901-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
93%
High Homology
Sheep
87%
Moderate Homology
Rabbit
86%
Moderate Homology
Cow
86%
Moderate Homology
Rat
85%
Moderate Homology
Mouse
84%
Moderate Homology
Zebrafish
49%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KDR antibody
Immunohistochemistry (IHC) protocol for KDR antibody
Publications
Application:
Species:
Luteolin ameliorates sympathetic stress-induced myocardial injury by inhibiting the VEGFA/VEGFR2 and PI3K/Akt/NF-kappaB signaling pathways
Published: 2026-08-17
Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
Application: WB
Impact Factor: 4.00
Species: Mouse,Human
PMID: 42604877
Design optimization of antibody-ligand motifs to enhance CAR-T redirection activity against solid tumors
Published: 2026-06-25
Journal: Cell Reports Medicine
Application: WB
Impact Factor: 14.00
Species: Human
PMID: 42349416
Dysregulated VEGF/VEGFR-2 Signaling and Plexogenic Lesions in the Embryonic Lungs of Chickens Predisposed to Pulmonary Arterial Hypertension
Published: 2024-04-19
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 4.90
Species: Chicken
PMID: 38674074
Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser
Published: 2023-11-07
Journal: Biomedical Journal
Application: WB,IHC
Impact Factor: 5.50
Species: Mouse,Human
PMID: 37944864
TIPE regulates VEGFR2 expression and promotes angiogenesis in colorectal cancer
Published: 2020-01-01
Journal: International Journal of Biological Sciences
Application: IHC
Impact Factor: 4.86
Species: Human
PMID: 31929755
Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro
Published: 2014-06-12
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 0.94
Species: Human
PMID: 25009634
A study of the Distribution and Density of the VEGFR-2 Receptor on Glioma Microvascular Endothelial Cell Membranes
Published: 2011-03-09
Journal: CELLULAR AND MOLECULAR NEUROBIOLOGY
Application: IF,AFM
Impact Factor: 2.42
PMID: 21547490
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Product Promise
We promise Anti-VEGF Receptor 2/KDR Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00901-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026