Anti-D-dopachrome decarboxylase DDT Antibody
Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Anti-D-dopachrome decarboxylase DDT Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-D-dopachrome decarboxylase DDT Antibody
See all DDT primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for DDT detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P. Reactive species: Human. DDT information: Molecular Weight: 12712 MW; Subcellular Localization: Cytoplasm .|
|Cite This Product||Anti-D-dopachrome decarboxylase DDT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1449)|
|Similar Products From
|Anti-D-dopachrome decarboxylase DDT Antibody may replace the following items: sc 86406|sc 86407.|
|Reactivity/Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human DDT(99-118aa RFFPLESWQIGKIGTVMTFL), different from the related mouse and rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For DDT (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1652|
|Species Of This Entry||Human|
|Protein Name||D-dopachrome decarboxylase|
|Alternative Names||DDT|DDCT; D-Dopachrome Decarboxylase; D-dopachrome tautomeraseDDCT; DDT; DOPD; EC 126.96.36.199; MIF2; Phenylpyruvate Tautomerase II|
|Post Tranlational Modifications||Phosphorylation; Hydroxylation; Oxidation; Reduction; Bromination; Cleavage; Nitration; Demethylation; Ubiquitination; Methylation; Nitrosylation; Deiodination; Dephosphorylation; Carboxylation; Deamination; Decarboxylation; Phosphorothionation; Ribosylation; Iodination; Acetylation|
|Gene Location||22q11.23, on Chromasome 22, gene sequence: NC_000022.11 (23971365..23979828, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For DDT (Source: Uniprot.org, NCBI)
|Protein Function||Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).|
|Research Areas||Stem Cell Markers|
|Related Diseases||Malaria; Malignant Neoplasms; Neoplasms; Poisoning; Malignant Neoplasm Of Breast; Mammary Neoplasms; Maternal Exposure; Liver Neoplasms; Malaria, Falciparum; Carcinogenesis; Nervousness; Carcinoma; Neoplasms, Experimental; Infective Disorder; Leishmaniasis; Liver Neoplasms, Experimental; Leishmaniasis, Visceral; Congenital Abnormality|
|Related Pathways||Transport; Insecticide Resistance; Ddt Resistance; Excretion; Lactation; Pyrethroid Resistance; Secretion; Cell Proliferation; Cell Death; Hatching; Cell Cycle; Aging; Fertilization; Immune Response; Localization; Mating; Response To Ddt; Pathogenesis; Flight; Spermatogenesis|
|Background||DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.|
|Scientific References||PMID: 9480844 by Nishihira J., et al. Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation.|
PMID: 9716662 by Esumi N., et al. Conserved gene structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.
Our Boster Quality Guarantee for Anti-D-dopachrome decarboxylase DDT Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDT Antibody (PA1449) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # PA1449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13KD. The expected band size for DDT is at 13KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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