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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-MMP9 Antibody Picoband™
Boster Bio Anti-MMP9 Antibody Picoband™ catalog # PB10008. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10008)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of rat MMP-9 (622-658aa RRGGKALLISRERIWKFDLKSQKVDPQSVTRLDNEFS), different from the related human sequence by nineteen amino acids, and from the related mouse sequence by nine amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB10008 is reactive to Mmp9 in Mouse, Rat
PB10008 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB10008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: NRK whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB10008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa, 92 kDa. The expected band size for MMP-9 is at 79 kDa.
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Figure 2. IHC analysis of MMP-9 using anti-MMP-9 antibody (PB10008).
MMP-9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB10008) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of MMP-9 using anti-MMP-9 antibody (PB10008).
MMP-9 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB10008) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Gene/Protein Information For Mmp9 (Source: Uniprot.Org, NCBI)
peptidase M10A family
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 188.8.131.52; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase Mmp9|matrix metallopeptidase 9|matrix metalloproteinase-9|92 kDa gelatinase|92-kDa type IV collagenase|GELB|MMP-9|gelatinase B|matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Mmp9, check out the Mmp9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mmp9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB10008 has been cited in 44 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Kefeng Zhai, Hong Duan, Wei Wang, Siyu Zhao, Ghulam Jilany Khan, Mengting Wang, Yuhan Zhang, Kiran Thakur, Xuemei Fang, Chao Wu, Jianbo Xiao, Zhaojun Wei,Ginsenoside Rg1 ameliorates blood–brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release, Acta Pharmaceutica Sinica B,2021,ISSN 2211 3835,https://doi.org/10. 1016/j.apsb.2021.03.032.
PB10008 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:NA
Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae. X. Jian,Xiao Chunhong,C. Hongyan,Lu Huijun,Yu Haizhong,Y. Jianfen
PB10008 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Zhao HM,Jin L,Liu Y,Hong X.Changes in expressions of miR-22-3p and MMP-9 in rats with thoracic aortic aneurysm and their significance.Eur Rev Med Pharmacol Sci.2020 Jun;24(12):6949-6954.doi:10.26355/eurrev_202006_21686.PMID:32633388.
PB10008 usage in article: APP:IHC, SAMPLE:HEART TISSUE, DILUTION:NA
Wang F,Ji S,Wang M,Liu L,Li Q,Jiang F,Cen J,Ji B.HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway.Eur J Pharmacol.2020 Aug 5;880:173189.doi:10.1016/j.ejphar.2020.173189.Epub 2020 May 15.PMID:32417325.
PB10008 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Liang Q,Lin Q,Li Y,Luo W,Huang X,Jiang Y,Qin C,Nong J,Chen X,Sooranna SR,Pinhu L.Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model.J Immunol Res.2020 Nov 25;2020:6644687.doi:10.1155/2020/6644687.PM
1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines
MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway
Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9
Early changes in the apparent diffusion coefficient and MMP-9 expression of a cervical carcinoma U14 allograft model following irradiation
Mesenchymal stem cells overexpressing adrenomedullin improve heart function through antifibrotic action in rats experiencing heart failure
Adipose differentiation-related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation
Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats
Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21
Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization
Preventive Effects of Rhodiola rosea L on Bleomycin-Induced Pulmonary Fibrosis in Rats
Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression
MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Isolation and characteristics of CD133?/A2B5+ and CD133?/A2B5? cells from the SHG139s cell line
Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo
Effect of Ginkgo biloba extract on experimental cardiac remodeling
Expression of matrix metalloproteinases-8 and -9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement
Huoxue Rongluo?Tablet reduces matrix metalloproteinase-9 expression in infarcted brain tissue
Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma
Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...
Cheng Ys, Dai Dz, Dai Y. Acta Pharmacol Sin. 2009 Aug;30(8):1099-106. Doi: 10.1038/Aps.2009.104. Epub 2009 Jul 13. Isoproterenol Disperses Distribution Of Nadph Oxidase, Mmp-9, And Ppkcepsilon In The Heart, Which Are Mitigated By Endothelin Recept...
Yang Tq, Lu Xj, Wu Tf, Ding Dd, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Guo Lc, Zhang Y, Huang Yl, Zhou Yx, Du Zw. Cancer Sci. 2014 Mar;105(3):265-71. Doi: 10.1111/Cas.12351. Epub 2014 Feb 11. Microrna-16 Inhibits Glioma Cell Growth And Invasion T...
Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...
Song Y, Zou H, Wang G, Yang H, Xie Z, Bi J. Neural Regen Res. 2012 Jun 15;7(17):1325-30. Doi: 10.3969/J.Issn.1673-5374.2012.17.007. Matrix Metalloproteinase-9 And Tissue Inhibitor Of Metalloproteinase-1 Expression In Early Focal Cerebral Infarctio...
Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...
Liu J, Ping W, Zu Y, Sun W. Int J Clin Exp Pathol. 2014 Aug 15;7(9):6040-7. Ecollection 2014. Correlations Of Lysyl Oxidase With Mmp2/Mmp9 Expression And Its Prognostic Value In Non-Small Cell Lung Cancer.
Chen Lx, He Yj, Zhao Sz, Wu Jg, Wang Jt, Zhu Lm, Lin Tt, Sun Bc, Li Xr. Cancer Biol Ther. 2011 Jan 15;11(2):229-35. Epub 2011 Jan 15. Inhibition Of Tumor Growth And Vasculogenic Mimicry By Curcumin Through Down-Regulation Of The Epha2/Pi3K/Mmp Pat...
Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Bear...
Ma X, Ma X, Ma Z, Sun Z, Yu W, Wang J, Li F, Ding J. Evid Based Complement Alternat Med. 2014;2014:710870. Doi: 10.1155/2014/710870. Epub 2014 Oct 15. The Effects Of Uygur Herb Hyssopus Officinalis L. On The Process Of Airway Remodeling In Asthmat...
Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...
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