Anti-Vimentin Antibody

Vimentin antibody

Boster Bio Anti-Vimentin Antibody catalog # M00235-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00235-4
Size: 100ul
Reactive Species: Bovine, Equine, Human, Mouse, Pig, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-Vimentin Antibody

View all Vimentin Antibodies

SKU/Catalog Number

M00235-4

Size

100ul

Description

Boster Bio Anti-Vimentin Antibody catalog # M00235-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-4)

Host

Rabbit

Contents

Antibody is supplied as an aliquot of serum

Clonality

Polyclonal

Immunogen

Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00235-4 is reactive to VIM in Bovine, Equine, Human, Mouse, Pig, Rat

Applications

M00235-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot: 1:10,000. IF/ICC and IHC: 1:5,000.

Validation Images & Assay Conditions

Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)

Uniprot ID

P08670

Gene ID

7431

Gene Name

VIM

Full Name

Vimentin

Weight

53.652kDa

Superfamily

intermediate filament family

Alternative Names

FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VIM, check out the VIM Infographic

VIM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M00235-4 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression

A novel approach to the identification and enrichment of cancer stem cells from a cultured human glioma cell line

Liu S,Xin W,Lu Q,Tang X,Wang F,Shao W,Zhang Y,Qiu J,Hua K. Knockdown of lncRNA H19 suppresses endometriosis in vivo. Braz J Med Biol Res.2021 Feb 26;54(4):e10117.doi:10.1590/1414-431X202010117.PMID:33656053;PMCID:PMC7917710.
Species: Human,Mouse
M00235-4 usage in article: APP:ICC, SAMPLE:ECESCS, DILUTION:1:50

Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
Species: Human,Mouse
M00235-4 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800

Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
M00235-4 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
Species: Human
M00235-4 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100

The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties

Expression of E-cadherin and vimentin in oral squamous cell carcinoma

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5 Customer Q&As for Anti-Vimentin Antibody

Question

Our lab used your anti-Vimentin antibody for WB on lymphoblast last year. I am using bovine, and I plan to use the antibody for IHC next. My lab would like examining lymphoblast as well as cervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

I have checked the website and datasheets of our anti-Vimentin antibody and I see that M00235-4 has been validated on bovine in both WB and IHC. Thus M00235-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-05-01

Question

Our team were happy with the WB result of your anti-Vimentin antibody. However we have observed positive staining in lymphoblast cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

From what I have seen in literature, lymphoblast does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2020-04-09

Question

We have observed staining in rat brain. What should we do? Is anti-Vimentin antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2018-03-30

Answer

Based on literature brain does express VIM. Based on Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2018-03-30

Question

My lab would like using your anti-Vimentin antibody for negative regulation of neuron projection development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

Thank you for your inquiry. This M00235-4 anti-Vimentin antibody is tested on hela cells. It is guaranteed to work for IHC, WB in bovine, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-01-29

Question

We are currently using anti-Vimentin antibody M00235-4 for bovine tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it likely that the antibody can work on dog tissues as well?

M. Huang

Verified customer

Asked: 2017-02-09

Answer

The anti-Vimentin antibody (M00235-4) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-02-09

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M00235-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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