Product Info Summary
SKU: | M00235-4 |
---|---|
Size: | 100ul |
Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Vimentin Antibody
SKU/Catalog Number
M00235-4
Size
100ul
Description
Boster Bio Anti-Vimentin Antibody catalog # M00235-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-4)
Host
Rabbit
Contents
Antibody is supplied as an aliquot of serum
Clonality
Polyclonal
Immunogen
Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00235-4 is reactive to VIM in Bovine, Equine, Human, Mouse, Pig, Rat
Applications
M00235-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53.652kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1:10,000. IF/ICC and IHC: 1:5,000.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.
Click image to see more details
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green, and mouse mAb to Actin, dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Protein Target Info & Infographic
Gene/Protein Information For VIM (Source: Uniprot.org, NCBI)
Gene Name
VIM
Full Name
Vimentin
Weight
53.652kDa
Superfamily
intermediate filament family
Alternative Names
FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIM, check out the VIM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vimentin Antibody (M00235-4)
Hello CJ!
M00235-4 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression
A novel approach to the identification and enrichment of cancer stem cells from a cultured human glioma cell line
Liu S,Xin W,Lu Q,Tang X,Wang F,Shao W,Zhang Y,Qiu J,Hua K. Knockdown of lncRNA H19 suppresses endometriosis in vivo. Braz J Med Biol Res.2021 Feb 26;54(4):e10117.doi:10.1590/1414-431X202010117.PMID:33656053;PMCID:PMC7917710.
Species: Human,Mouse
M00235-4 usage in article: APP:ICC, SAMPLE:ECESCS, DILUTION:1:50
Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
Species: Human,Mouse
M00235-4 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
M00235-4 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
Species: Human
M00235-4 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties
Expression of E-cadherin and vimentin in oral squamous cell carcinoma
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
5 Customer Q&As for Anti-Vimentin Antibody
Question
Our lab used your anti-Vimentin antibody for WB on lymphoblast last year. I am using bovine, and I plan to use the antibody for IHC next. My lab would like examining lymphoblast as well as cervix in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I have checked the website and datasheets of our anti-Vimentin antibody and I see that M00235-4 has been validated on bovine in both WB and IHC. Thus M00235-4 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-01
Question
Our team were happy with the WB result of your anti-Vimentin antibody. However we have observed positive staining in lymphoblast cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
From what I have seen in literature, lymphoblast does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2020-04-09
Question
We have observed staining in rat brain. What should we do? Is anti-Vimentin antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
Based on literature brain does express VIM. Based on Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579
Boster Scientific Support
Answered: 2018-03-30
Question
My lab would like using your anti-Vimentin antibody for negative regulation of neuron projection development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you for your inquiry. This M00235-4 anti-Vimentin antibody is tested on hela cells. It is guaranteed to work for IHC, WB in bovine, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-29
Question
We are currently using anti-Vimentin antibody M00235-4 for bovine tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, equine, human, mouse, pig, rat. Is it likely that the antibody can work on dog tissues as well?
M. Huang
Verified customer
Asked: 2017-02-09
Answer
The anti-Vimentin antibody (M00235-4) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-09