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Product Info Summary
| SKU: | PB9956 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband®
SKU/Catalog Number
PB9956
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband® catalog # PB9956. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. ORM1 (α1-acid glycoprotein) functions as a bloodstream transport protein binding diverse ligands/drugs and is associated with the acute-phase response (well-established). Assay context: primary antibody reactive with human/mouse/rat, validated for ELISA, IHC, and WB on the product page. In inflammation studies (putative), systemic ORM1 protein is often interpreted alongside innate activation (e.g., TLR2 ) and proteolysis/migration markers such as uPAR/PLAUR; transcript–protein concordance can be supported by qPCR (methodological), while tissue localization aligns with IHC.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9956)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ORM1 recombinant protein (Position: Q19-S201). Human ORM1 shares 47.5% and 48.1% amino acid (aa) sequence identity with mouse and rat ORM1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9956 is reactive to ORM1 in Human, Mouse, Rat
Observed Molecular Weight
40-47 kDa
Calculated molecular weight
23.5 kDa
Background of ORM1
Alpha-1-acid glycoprotein 1 is a protein that in humans is encoded by the ORM1 gene. The structural gene for orosomucoid (ORM1) is assigned to the end of the long arm of chromosome 9 by demonstration of linkage to ABO and AK1. This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9956 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HCCT tissue, human HCCP tissue, rat liver tissue, mouse liver tissue
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
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IHC analysis of ORM1 using anti-ORM1 antibody (PB9956).
ORM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ORM1 Antibody (PB9956) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband® (PB9956)
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5 Customer Q&As for Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband®
Question
Does anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
According to the expression profile of plasma, ORM1 is highly expressed in plasma. So, it is likely that anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 will work for WB with plasma.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use your anti-Alpha 1 Acid Glycoprotein/ORM1 antibody for WB for rat plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat plasma identification?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
It shows on the product datasheet, PB9956 anti-Alpha 1 Acid Glycoprotein/ORM1 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-02
Question
Would anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 work on primate ELISA with liver?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Our lab technicians have not validated anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
We are currently using anti-Alpha 1 Acid Glycoprotein/ORM1 antibody PB9956 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
D. Jones
Verified customer
Asked: 2016-06-09
Answer
The anti-Alpha 1 Acid Glycoprotein/ORM1 antibody (PB9956) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-09
Question
Will PB9956 anti-Alpha 1 Acid Glycoprotein/ORM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Miller
Verified customer
Asked: 2013-08-14
Answer
You can see on the product datasheet, PB9956 anti-Alpha 1 Acid Glycoprotein/ORM1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-14
