Anti-Von Willebrand Factor/VWF Antibody Picoband®

IHC analysis of VWF using anti-VWF antibody (PB9062).
VWF was detected in paraffin-embedded section of Human Lung Cancer Tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9062) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of VWF using anti-VWF antibody (PB9062).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HT1080 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VWF antigen affinity purified polyclonal antibody (Catalog # PB9062) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VWF at approximately 309KD. The expected band size for VWF is at 309KD.

IHC analysis of VWF using anti-VWF antibody (PB9062).
VWF was detected in frozen section of human placenta tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9062) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Flow Cytometry analysis of A431 cells using anti-VWF antibody (PB9062).
Overlay histogram showing A431 cells stained with PB9062 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VWF Antibody (PB9062, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Effects of TMP on the cytoskeleton in vitro . (A) VWF staining was used to identify endothelial cells (scale bar = 50 μm). (B) Transwell chamber FITC albumin was used to determine the effect of TMP (15 ng/ml) on HUVEC after 12 h of LPS (200 ng/ml) exposure. (C) Immunofluorescence assay for the cytoskeleton (scale bar = 50 μm). (D) Transendothelial electrical resistance (TEER) in TMP-treated, untreated, and control HUVECs. * p < 0.05, ** p < 0.01, n = 3 per group.
Index in PubMed under a CC BY license. PMID: 36686718

H&E, Masson’s trichrome stain, and IHC examinations of the retrieved tissue and the tendon. ( A ) H&E stain; ( B ) Masson’s trichrome stain; ( C ) VEGF; ( D ) BMP-2; ( E ) TGF-β; ( F ) vWF; ( G ) collagen I; ( H ) collagen III.
Index in PubMed under a CC BY license. PMID: 38081952