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VWF antibody (PB9062) performs well in Flow Cytometry, IHC, IHC-F, ICC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 21 Publications

Flow Cytometry analysis of A431 cells using anti-VWF antibody (PB9062).
Overlay histogram showing A431 cells stained with PB9062 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VWF Antibody (PB9062, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Von Willebrand Factor/VWF Antibody Picoband®

VWF antibody

21 Publications

Reactive Species	Host	Application
Human	Rabbit	Flow Cytometry, IHC, IHC-F, ICC, WB

Tested Applications

VWF antibody was positively detected by WB in:

HT1080 Whole Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

VWF antibody was positively detected by IHC in:

Human Lung Cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

VWF antibody was positively detected by IHC-F in:

human placenta tissue

VWF antibody was positively detected by FC in:

A431 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Von Willebrand Factor/VWF Antibody Picoband®
View all VWF Antibodies

Form

Lyophilized

Uniprot

P04275

Gene ID

7450

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

309 kDa

Calculated Molecular Weight

309.3 kDa

Background of VWF

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Cite This Product

Anti-Von Willebrand Factor/VWF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9062)

Protocols

Western Blot protocol for VWF antibody

Review Protocol

Immunohistochemistry (IHC) protocol for VWF antibody

Review Protocol

Flow Cytometry (FC) protocol for VWF antibody

Review Protocol

Publications

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Published: 2023-12-11

Journal: Scientific Reports

Application: IHC

Impact Factor: 4.60

Species: Rat

PMID: 38081952

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Anti-Von Willebrand Factor/VWF Antibody Picoband®

VWF antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Novel CO2-encapsulated Pluronic F127 hydrogel for the treatment of Achilles tendon injury

Anti-Adhesive Resorbable Indomethacin/Bupivacaine-Eluting Nanofibers for Tendon Rupture Repair: In Vitro and In Vivo Studies

Electrospun Nanofibrous Composite Membranes for Long-Term Release of Celecoxib for Achilles Tendon Reconstruction Following Injury

Tetramethylpyrazine ameliorates acute lung injury by regulating the Rac1/LIMK1 signaling pathway

A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure

Tri-Layered Doxycycline-, Collagen- and Bupivacaine-Loaded Poly(lactic-co-glycolic acid) Nanofibrous Scaffolds for Tendon Rupture Repair

The protective effects of angelica organic acid against ox-LDL-induced autophagy dysfunction of HUVECs

Urethra-inspired biomimetic scaffold: A therapeutic strategy to promote angiogenesis for urethral regeneration in a rabbit model

Homocysteine induced oxidative stress in human umbilical vein endothelial cells via regulating methylation of SORBS1

Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senescence through SIRT1

The effect and action mechanism of resveratrol on the vascular endothelial cell by high glucose treatment

IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells

RoY Peptide-Modified Chitosan-Based Hydrogel to Improve Angiogenesis and Cardiac Repair under Hypoxia

A ferulic acid (FA)-eluting system for biodegradable magnesium stent: Cells response of HUVECs

Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells

Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro

Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury.

Role of axonal guidance factor netrin-1 in human placental vascular growth

Identification and characterization of cancer stem-like cells from primary carcinoma of the cervix uteri

PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

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